Degussa Aktiengesellschaft

Forschungszentrum Juelich Gmbh

Other

Forschungszentrum Julich Gmbh

Kernforschungsanlage Julich Gesellschaft MIT Beschrankter Haftung

Kernforschungsanlage Julich

Bayer Aktiengesellschaft

Ciba-geigy Corporation

Dsm IP Assets B.v.

Schott Glaswerke

Forschungszentrum J&uuml;lich Gmbh

F. Hoffmann-la Roche Ag

Christian Wandrey

Udo Kragl

Maria-Regina Kula

Wolfgang Leuchtenberger

Alexander Aivasidis

Karlheinz Drauz

Andreas Liese

Manfred Biselli

Rolf Wichmann

Alexander Schwarz

Andreas Bommarius

Oreste Ghisalba

Andreas Buckmann

Savas Anastassiadis

Doerte Steinke

Werner Kiefer

Werner Hummel

Andreas Sebastian Bommarius

Claus Dreisbach

Guido Giffels

Guenter Knaup

Olaf Burkhardt

Ulrich Groeger

Hans Henniges

Daniel Gygax

Matthias Kittelmann

Carsten Bolm

Andreas Karau

Jean-Louis Philippe

Kurt Hamacher

Thomas Link

Peter Miethe

Hans-Joachim Schmoll

Jens Woeltinger

Karl-Heinz Drauz

Nadine Derrien

Heinz Hubert Coenen

Toshihisa Ohshima

Michael M&uuml;ller

Michael Wolberg

Manfred Kircher

Eberhard Breuker

Dirk Weuster-Botz

Andreas Fischer

Christoph Born

Bernd Scr Oder

Wolfgang Kruse

Murillo De Oliveira Villela Filho

Bernd Schroder

Rainer Pick

Thomas Tierling

Jan A M De Bont

Juliane Beliczey

Jan C Verdoes

Marcel Felder

Carel A G M Weijers

Klaus Memmert

Hans-Juergen Schuetz

J Hans Visser

Bernhard Muetze

August W Alfermann

Ralf Takors

Nagaraj N Rao

Maike Petersen

Jochen Gaetgens

Dietrich H Maass

Maria R Kula

Uwe Neidermeyer

Holger Paschold

Kyriakos Makrylaleas

Joerg Thoemmes

Stephan Laue

Ingeborg M Stuertz

Teresa Klein

Thomas Noll

Ruth Essers

Kerstin Skopnik

Jochen Gätgens

The present invention relates to a process for the kinetic resolution of racemic functionalized epoxides in the presence of microorganisms, crude or pure preparations thereof comprising a polypeptide having epoxide hydrolase activity. Preferred microorganisms are yeasts and bacteria which may also be recombinant.

Process for the stereoselective preparation of functionalized vicinal diols

The present invention relates to a process for separating from an aqueous mixture one or more organic substances containing at least one positively charged and/or chargeable nitrogenous group by means of extraction via at least one porous membrane, wherein use is made of an extraction agent which contains at least partially relatively long-chain organic compounds and at least one liquid cation exchanger, and of a membrane that is wettable by either the aqueous mixture or by the extraction agent. In particular, the invention relates to a process for separating from an aqueous mixture one or more organic substances containing at least one positively charged and/or chargeable nitrogenous group by means of extraction via at least one porous membrane, wherein the aqueous mixture is drawn from a reservoir , led across a first porous membrane which is wettable by either the aqueous mixture or by an extraction agent which contains at least partially relatively long-chain organic compounds and at least one liquid cation exchanger, extracted with the extraction agent , the aqueous retentate is returned to the reservoir, the extracted organic substances are led across a second porous membrane which is wettable by either the aqueous mixture or by the extraction agent and there re-extracted into an aqueous phase

Process for the separation of organic substances from an aqueous mixture

Use of molecular weight-enlarged catalysts in a process for asymmetric, continous hydrogenation, novel molecular weight-enlarged ligands and catalysts

In a method of reducing diketocarboxylic acids or hydroxyketocarboxylic acids or their esters, at least one keto group is converted to a hydroxyl group in the presence of lactobacillus species.

Method of reducing keto-carboxylic acids and their esters

Furanosides or pyranosides can be reacted with pyruvate and converted into sialic acids in good yields by an enzymatic synthesis, using a suitable aldolase, where the concentration of aldolase, per 50 mM of pyruvate, is 1 to 2,500 U*/ml. For example, 3-fluoroneuraminic acid and other 3,3-didesoxy-3-fluorononulopyranosonic acid derivatives can be prepared in this manner. The F-atom in the resulting sialic acid (which can be F or F and F) provides a label which makes possible studies of mammalian physiology and diagnosis of mammalian diseases.

Method for making fluorinated sugars having a side chain and use thereof

A process for producing citric acid/citrate for use in producing citric acid, comprising the steps of continuously feeding to a fermenter containing a yeast capable of transforming glucose to citric acid by fermentation, in the presence of a nutrient medium and oxygen, sugar, especially glucose as a C source and at least one ammoniun compound as an N source fermenting the sugar to citric acid in said fermenter at a temperature, a pH and with a residence time sufficient to transform at least a major part of the sugar continuously fed to said fermenter to citric acid continuously withdrawing fermentation product from said fermenter recovering citric acid/citrate from said fermentation product controlling a C/N ratio fed to said fermenter to correspond to a molar ratio of glucose to ammonium compounds of 12 to 22 during fermentation of the glucose to citrate.

Process for the continuous production of citric acid by fermentation

The invention involves a procedure for the catalytic enantioselective reduction of ketones to chiral alcohols. Heretofore, this reaction was carried out batch-wise. Because, in this procedure, one utilizes a catalyst increased in size with polymer, it is possible to carry out the reaction in a quasi-continuous manner in a membrane reactor. In contrast to the state of the art, one thus dramatically increases the operative life of the catalyst.

Process for the catalytic enantioselective reduction of ketones

Strains of Aureobasidium pullulans (de bary) Arnaud can be used for the commercial production of gluconic acid by fermentation in aqueous liquid containing sugar, which in continuous culture from glucose form greater than or equal to 90% and greater than or equal to 90% molar selectivity. The process is conducted with an Fe and Mn optimized medium with a nitrogen-independent (N-independent) ion concentration. The iron (Fe) concentration with 3 g/l NH.sub.4 Cl is greater than or equal to 0.5 mM, and the manganese (Mn) concentration with 3 g/l NH.sub.4 Cl is greater than or equal to about 0.5 mM manganese (Mn). The pH is regulated between about 4.5 and about 8, in particular at 6.5-7, and the temperature between 24 and 32.degree. C., in particular at 29-30.degree. C. Particularly successful strains are Aureobasidium pullulans (de bary) Arnaud with the registration numbers DSM 7085, DSM 7086, DSM 7087, and DSM 7088.

Process for the production of gluconic acid with a strain of

Stem cells or other organ-function cells are cultivated in a fluidized bed system on macroporous glass carrier bodies treated with a structure protein such as gelatin and an extracellular matrix protein such as fibronectin, and coated with a stroma cell layer. Glass carriers coated with gelatin are added to a fluidized bed reactor, and a culture medium containing an extracellular matrix protein is added to bind the protein to the gelatin. Stromal cells are then added and the cells are cultured to immobilize the cells on the carriers containing the bound protein. Immature organ-function cells are added to the reactor, and while generating a fluidized bed of the carriers in the culture medium, the culture medium is recirculated from and to the reactor in a recirculation loop. Bubble-free aeration of the culture medium is effected to cultivate the immature organ-function cells on the carriers to obtain both mature differentiated organ-function cells and progenitor organ-function cells. Mature differentiated organ-function cells which are released from the carriers are harvested from the reactor while retaining the progenitor organ-function cells which adhere to the carriers to provide an equilibrium between the harvested mature differentiated organ-function cells, the progenitor cells and the immature cells added to the reactor. Growth and regulatory factors for cultivating organ-function cells can be produced by culturing immobilized stromal cells in the reactor in the absence of the organ-function cells.

Culturing cells on macroporous glass carriers coated with gelatin,

In a process for the continuous enzyme-catalyticzed enzymatic production of hydrophobic products in an aqueous solution of a reaction mixture, the product is extracted from the product containing reaction mixture by way of a product-permeable microporous membrane into an organic solvent and the product-depleted reaction mixture is recycled.

Process for the continuous enzymatic extraction of hydrophobic products

A cell culture reactor can have one or more gasification modules in which tubular membrane hoses surround rigid inner tubes which extend above the membranes to communicate with gas inlets or outlets. The tube is anchored in a closure member at the lower end of the membrane.

Gasification-tube module and reactor for cell cultivation

A peptide amidase isolated from the flavedo of citrus fruits, preferably oranges, which is capable of catalyzing the selective hydrolytic elimination of the free amino group on the C-terminal end of peptide amides but which does not cleave peptide bonds. The enzyme accepts D-amino acid residues in the C-terminal position, although the hydrolysis rate is much slower than with L-amino acid residues. The enzyme is weakly inhibited by serine protease inhibitors; has an optimal pH of 7.5.+-.1.5, an optimum temperature of 30.degree. C. at pH 7.5 and has an isoelectric point of pH 9.5. The peptide amidase is stable at pH 6.0-9.0. The molecular weight of the purified enzyme is 32,000.+-.3000 daltons. A peptide amidase according to the present invention is particularly useful in the production of peptides by continuous enzymatic reaction of N-protected amino acid or peptide alkyl esters with amides of amino acids. In the continuous reaction, the synthesized peptide amide is hydrolyzed by the peptide amidase and separated by anion exchange from the amide of the amino acid which can be recycled.

Peptide amidase and the use thereof

The invention relates to a process for the production of cytidine 5'-monophosphosialic acids which comprises reacting a sialic acid with cytidine 5'-triphosphate in the presence of a cell extract of a naturally occurring microorganism having cytidine 5'-monophospho-N-acetylneuraminic acid synthetase activity, the extract optionally having been subjected to one purification step.

Process for the production of activated sialic acids

Protected and unprotected di- or oligopeptides are synthesized by reacting an N-terminally protected .alpha.-amino acid alkyl ester or peptide alkyl ester of the formula X--E--R.sup.1 with an amino acid or a di- or oligopeptide or a derivative thereof of the formula H.sub.2 N--Q--R.sup.2 in aqueous solution in the presence of a hydrolase, and, removing protective groups from the reaction product separated from the reaction mixture, where E is the residue of an .alpha.-amino acid or of a di- or oligopeptide, R.sup.1 is lower alkyl and X is a group which carries a charge or is polar at the pH values used for the reaction and which increases the solubility by a factor &gt;5 compared with compounds wherein X=H, Q is the residue of an amino acid or of a di- or oligopeptide, and R.sup.2 is an optionally esterified or amidated acid group. In a preferred embodiment, the peptide or (.alpha.-)amino acid ester concentration is greater than 50 mM, wherein in a particularly preferred embodiment, the concentration ranges from 100 to 1000 mM with approximately comparable nucleophile and electrophile concentrations. The preferred substrate/enzyme ratios are &gt;10.sup.5 M/M or &gt;10.sup.3 M/M when papain is employed. Phthalyl, maleyl or citraconyl radicals and their derivatives or N-betainyl compounds are envisaged for use radicals (X) in the electrophilic component.

Process for the enzymatic preparation of protected and unprotected di-

The invention is directed to a method of producing L-amino acids from .alpha.-ketocarboxylic acids by means of continuous fermentation with the aid of glutamate producing bacteria with biomass retention in which the cells are separated by microfiltration and/or centrifugal separators.

Method for the fermentative production of L-amino acids from

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Juelich, Germany

Christian Wandrey