Leica Microsystems Cms Gmbh

Christian Schumann

Christian Schulz

Alexander Weiss

Tobias Bauer

Ronja Capellmann

Andreas Lotter

Kai Ritschel

Benjamin Deissler

Arnold Mueller-Rentz

Sebastian Hitzler

Mate Beljan

Cornell Gonschior

Florian Fahrbach

Frank Sieckmann

Albrecht Weiss

Peter Euteneuer

Ralf Krueger

Patric Pelzer

Wernher Fouquet

Klaus Hermanns

Oliver Schlicker

Arnold Müller-Rentz

Theo Larrieu

Gheorghe Cojoc

Andrea Muelter

Alexander Weiß

Ralf KRÜGER

A method for examining a sample containing a target fluorophore j using a fluorescence microscope includes acquiring a series of sample images over an acquisition time interval, and adjusting an illumination parameter Pin a plurality of iteration steps n during the acquisition time interval to different set values. The series of sample images are acquired after adjusting the illumination parameter Pin at least some of the plurality of iteration steps n. The method further includes determining a bleaching behaviour descriptor κindicative of a bleaching behaviour of the target fluorophore j and a fluorescence response descriptor Iindicative of a fluorescence response of the target fluorophore j for the set values of the illumination parameter P. The illumination parameter Pis adjusted based on the bleaching behaviour descriptor κand the fluorescence response descriptor Ias determined in a preceding one of the plurality of iteration steps n.

Method for examining a fluorescent sample, microscope system and computer program

A light sheet microscope includes a light source configured to emit illumination light, an optical system configured to form a light sheet from the illumination light in a sample space, the light sheet being focused in a thickness direction perpendicular to a light propagation direction thereof to form a beam waist in the thickness direction, wherein the optical system has a field diaphragm adjustable to vary a width of the light sheet in a width direction, a scanning element configured to move the light sheet a scanning distance in the sample space along a scanning direction, and a control unit configured to control the field diaphragm for adjusting the width of the light sheet and to control the scanning element for moving the light sheet by the scanning distance to manipulate a target area of a sample by scanning the target area with the beam waist.

Light sheet microscope and method for manipulating a target area of a sample

A microscope for imaging a sample by a transmitted light contrasting method includes an objective lens holder configured to place an objective lens of a number of objective lenses onto an optical axis of the microscope. The microscope further includes a lens system for forming an intermediate image of an exit pupil of any one of the number of objective lenses placed onto the optical axis. The intermediate image is formed at a respective conjugated plane conjugate to the exit pupil. The microscope further includes a control device configured for automatically positioning a modulation element onto the optical axis at a positon related to the respective conjugated plane.

Microscope for transmitted light contrasting

An oblique plane microscope includes an optical imaging system configured to form an image of an object. The optical imaging system includes a telescope system with an optical zoom system, which is adjustable for adapting a magnification of the telescope system to a ratio between two refractive indices, one of which being associated with an object side of the telescope system and the other being associated with an image side of the telescope system. The oblique plane microscope further includes a control unit. The control unit is configured to evaluate an image quality of the image formed by the optical imaging system and to adjust the optical zoom system based on the evaluation.

Oblique plane microscope and method for correcting an aberration in an oblique plane microscope

An optical system for a microscope for imaging an object includes: a telescope system having an optical correction unit, which is adjustable in order to correct a spherical imaging aberration, and having a zoom optical unit, which is adjustable in order to adapt a magnification of the telescope system to a ratio of two refractive indices, one of which is assigned to an object side and an other of which is assigned to an image side, within a predetermined magnification range. The telescope system is telecentric over an entire magnification range both with respect to the object side and with respect to the image side by the zoom optical unit contained in the telescope system.

Optical system for a microscope

A method of analyzing a mixed fluorescence response of a plurality of fluorophores in a microscopic sample includes reconstructing individual fluorescence responses from a mixed fluorescence response using spectral un-mixing based on reference emission spectra for fluorophores to be reconstructed, and a procedure for determining and validating reference emission spectra including providing a plurality of image acquisition settings for a sequence of images of the sample equal to, or greater than, the plurality of fluorophores and including an illumination setting for each image, acquiring the sequence of images using the plurality of image acquisition settings and storing each image together with the corresponding illumination setting, determining candidate reference emission spectra for the fluorophores to be reconstructed from the sequence of images of the sample using one or more reference emission spectra determination algorithms, and conditionally using the candidate reference emission spectra as the reference emission spectra in the spectral un-mixing.

Method of analyzing a mixed fluorescence response of a plurality of fluorophores, fluorescence analyzer, fluorescence microscope and computer program

An optical system for a light-sheet microscope comprises transporting optics configured to project, into a sample, a light sheet for illuminating a sample plane positioned obliquely to an optical axis of the transporting optics and to project the illuminated sample plane into an intermediate image space. The transporting optics comprises an interchanging system that includes a first light-deflection element and a second light-deflection element. The interchanging system is configured to switch an illumination direction along which the light sheet illuminates the sample by alternately introducing the first light-deflection element and the second light-deflection element into a beam path of the transporting optics. The first light-deflection element causes a partial image inversion in only one direction. The second light-deflection element causes a complete image inversion in two directions.

Optical system for a light sheet microscope

A device for receiving samples in a microscope is provided. The device comprises a microtiter plate. The microtiter plate comprises an inner part with cavities for the samples and with an optically transparent bottom plate, which seals the cavities underneath and a frame which defines a supporting surface and is configured to hold the inner part at least in a first position. The bottom plate is arranged above the supporting surface in the first position of the inner part. The inner part is movable relative to the frame along a direction (A) perpendicular to the supporting surface.

Device for holding specimens in a microscope

A method for automatically ascertaining illumination brightnesses to be adjusted of at least two light sources for exciting at least one respective fluorophore in a sample to be imaged in a fluorescence microscope includes separately controlling, in terms of illumination brightness, each of the at least two light sources, detecting an image intensity of a microscopically imaged sample with at least two detectors, and automatically ascertaining the illumination brightnesses to be adjusted of the at least two light sources in such a way that a predefined setpoint of a signal-to-noise ratio is reached for each fluorophore. In order to ascertain the illumination brightnesses of the at least two light sources, cross-talk of a detector for different emission spectra of the fluorophores and/or cross-excitation of a fluorophore for different illumination spectra of the light sources are/is taken into account.

Method for adjusting the illumination in a fluorescence microscope, and corresponding fluorescence microscope

A microscope is provided. The microscope includes a lens system comprising a lens unit, which is adjustable along an optical axis of the lens system to correct an imaging error. The microscope further includes a motor-actuatable adjustment device, which is configured to adjust the lens unit along the optical axis. The microscope also includes a processor and a scanning unit, which is configured to deflect a light beam used for the image recording. The processor is configured to compare a position of an image which has been recorded after a correction adjustment of the lens unit to reference data, detect a change of the position of the image due to the correction adjustment of the lens unit based on the comparison, and activate the scanning unit in such a way that the change of the position of the image is at least partially compensated for.

Microscope and method for operating a microscope

A multispectral microscope system includes a first detector element for capturing a first image of a sample in a first spectral channel, and at least a second detector element for capturing a second image of the sample in a second spectral channel. The first detector element includes a first detector array. The second detector element includes a second detector array different from the first detector array. The microscope system further includes a processor for determining a spatial correlation between the first and second images based on a spectral crosstalk between the first and second spectral channels and registering the first and second images based on the spatial correlation.

Multispectral microscope system and method for registering first and second images by means thereof

An optical imaging device for a microscope includes an objective and an optical system configured to interact with the objective for optically imaging an object selectively in a first operating mode and a second operating mode. The optical system includes a first optical subsystem associated with the first operating mode, and a second optical subsystem associated with the second operating mode. The first optical subsystem is configured to form a first image of the object with a first magnification. The second optical subsystem is configured to form a second image of the object with a second magnification that is less than the first magnification. The second optical subsystem includes an optical module insertable into the optical path for selecting the second operating mode. The optical module includes a lens element with a positive refractive power.

Device and method for imaging an object

A microscope includes: a motorized object stage for moving an object; an optical imaging system for forming an optical image of a plane (OE) in which the object is to be optically imaged; an optical scanning unit for moving the plane (OE) to be optically imaged by the optical imaging system relative to the optical imaging system; an image sensor for detecting the optical image of the plane (OE) formed by the optical imaging system; and a controller for controlling the motorized object stage and the optical scanning unit for simultaneously moving the object and the plane (OE) in a same direction relative to the optical imaging system while the optical image is being detected by the image sensor.

Microscope and method for imaging an object using a microscope

A method is useable for correcting a spherical aberration of a microscope having an objective and a cover slip or object carrier, the objective having a correction element for correcting the spherical aberration. The method includes: ascertaining, by the microscope, an index of refraction of an optical medium bordering the cover slip or object carrier and/or a thickness of the cover slip or object carrier along an optical axis of the objective, ascertaining the spherical aberration based on the index of refraction of the optical medium and/or the thickness of the cover slip or object carrier, and ascertaining, based on the spherical aberration, a positioning variable, by which the correction element is adjusted so that the spherical aberration is corrected.

Method for correcting a spherical aberration of a microscope, and microscope

A fluorescence microscope, includes an optical system configured to collect fluorescent light emitted from different fluorophore species within a field of view and to focus the fluorescent light for detection, a spectral splitting arrangement configured to split the fluorescent light into at least two spectrally different fluorescent light components, a multi-channel detector system including at least two image sensors configured to detect at least two spatial light intensity distributions based on the at least two spectrally different fluorescent light components, each spatial light intensity distribution representing an image of the object over the field of view, and a processor configured to determine spatial distributions of the different fluorophore species based on a spectral unmixing analysis of each spatial light intensity distribution, wherein the processor is further configured to obtain compensation information and to determine a spatial distribution of each fluorophore species by taking into account the compensation information.

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Lich, Germany

Christian Schumann