Max-planck-gesellschaft Zur F&#xf6;rderung Der Wissenschaften E. V.

Christian Ringemann

Stefan W Hell

Volker Westphal

Christian Wurm

Christian Eggeling

Vladimir Belov

Kirill Kolmakov

Stefan Jakobs

Marcel Lauterbach

Christian A Wurm

The invention relates to novel and improved photostable rhodamine dyes of the general structural formulae I or II and their uses as fluorescent markers, e.g. for immunostainings and spectroscopic and microscopic applications, in particular in conventional and stimulated emission depletion (STED) microscopy and fluorescence correlation spectroscopy. The partially deuterated analogues are useful as molecular mass distribution tags in mass spectroscopic applications, wherein R=an unsubstituted or substituted alkyl group, including a cycloalkyl group, or heterocycloalkyl group; R=H, an unsubstituted or substituted alkyl group, including a cycloalkyl group, or heterocycloalkyl group, or an unsubstituted or substituted aryl group or heteroaryl group, or any combination of such groups; X=CH, C&#x2550;O, C&#x2550;NOR, C&#x2550;NNRNR, CH(OR), O, S, SO, SO, or any other derivatives of these groups, with Ra and Rb independently being H or an organic residue, in particular an unsubstituted or substituted (cyclo)alkyl group or heterocycloalkyl group, an unsubstituted or substituted aryl group or heteroaryl group; Z=a negatively charged group with 1, 2, 3, 4 or 5 charges per anion. 

Hydrophilic and lipophilic rhodamines for labelling and imaging

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Goettingen, Germany

Christian Ringemann