Joule Unlimited Technologies, Inc.

Joule Unlimited, Inc.

Christian Perry Ridley

Nikos Basil Reppas

Dan Eric Robertson

Frank Anthony Skraly

David Arthur Berry

Noubar Boghos Afeyan

Martha Sholl

Brian D Green

Sriram Kosuri

Regina Wilpiszeski

Jacob Carter Harrison

Michael Connor

Eric James Devroe

Brian Green

Eric Devroe

The present disclosure identifies methods and compositions for modifying photoautotrophic organisms as hosts, such that the organisms efficiently convert carbon dioxide and light into n-alkanes, and in particular the use of such organisms for the commercial production of n-alkanes and related molecules.

Methods and compositions for the recombinant biosynthesis of n-alkanes

The present disclosure identifies pathways and mechanisms to confer production of carbon-based products of interest such as ethanol, ethylene, chemicals, polymers, n-alkanes, isoprenoids, pharmaceutical products or intermediates thereof in photoautotrophic organisms such that these organisms efficiently convert carbon dioxide and light into carbon-based products of interest, and in particular the use of such organisms for the commercial production of ethanol, ethylene, chemicals, polymers, n-alkanes, isoprenoids, pharmaceutical products or intermediates thereof.

Engineered CO2 fixing microorganisms producing carbon-based products of interest

Various 1-alkenes, including 1-nonadecene and 1-octadecene, are synthesized by the engineered microorganisms and methods of the invention. In certain embodiments, the microorganisms comprise recombinant 1-alkene synthases. The engineered microorganisms may be photosynthetic microorganisms such as cyanobacteria.

Biosynthesis of 1-alkenes in engineered microorganisms

The present disclosure identifies methods and compositions for modifying photoautotrophic organisms as hosts, such that the organisms efficiently convert inorganic carbon and light into n-alkanes, and in particular the use of such organisms for the commercial production of n-alkanes and related molecules.

Photoalkanogens with increased productivity

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The present disclosure identifies methods and compositions for modifying photoautotrophic organisms, such that the organisms efficiently convert carbon dioxide and light into compounds such as esters and fatty acids. In certain embodiments, the compounds produced are secreted into the medium used to culture the organisms.

Methods and compositions for the recombinant biosynthesis of fatty acids and esters

The NonA alkene synthase insp. displays selective synthesis of 1-nonadecene. Heterologous recombination of a domain, i.e. the acyl binding domain, with other acyl binding proteins, affects acyl substrate chain-length binding selectivity and therefore the chain-length of the synthesized 1-alkenes. Compositions and methods are provided to selectively synthesize 1-alkenes of various chain lengths.

Methods and compositions for producing alkenes of various chain length

Methods and compositions for the recombinant biosynthesis of N-Alkanes

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Acton, MA, United States of America

Christian Perry Ridley