Nanogen Recognomics Gmbh

Degussa Aktiengesellschaft

Aventis Research & Technologies Gmbh & Co Kg

Aventis Research & Technologies Gmbh & Co.

Christian Miculka

Norbert Windhab

Tilmann Brandstetter

Gerhard Burdinski

Stefan Scherer

Thomas Wagner

Gerhard Quinkert

Hans-Ulrich Hoppe

Albert Eschenmoser

The invention relates to a process for preparing a conjugate that includes a pentopyranosyl nucleic acid and a biomolecule. The process includes the steps of providing a pentopyranosyl nucleic acid having at least two pentopyranosyl nucleotide subunits that are covalently linked between carbon 4 and carbon 2 of their respective pentopyranosyl rings. The pentopyranosyl nucleic acid also has an electrophilic reactive group. A biomolecule having a nucleophilic reactive group is also provided. The electrophilic reactive group of the pentopyranosyl nucleic acid and the nucleophilic reactive group of the biomolecule are reacted to form a covalent bond.

Process for preparing a pentopyranosyl nucleic acid conjugate

Provided are processes for preparing a 3'-deoxypentopyranosyl oligomers with linkers for linking biomolecules. The processes can the steps of: bonding a 4&#x2032;-protected-3&#x2032;-deoxypentopyranosyl nucleoside to a solid support by coupling the 2&#x2032;-OH group with a CPG support or other similar support with an amide linkage; deprotecting the 4&#x2032;-protected-3&#x2032;-deoxypentopyranosyl nucleoside at the 4&#x2032; position; deprotecting the 4&#x2032;-protected-3&#x2032;-deoxypentopyranosyl nucleoside at the 4&#x2032; position; and conjugating a linker to the free 4&#x2032; position. The resulting product can be conjugated via the linker to a biomolecule. The method can include, prior to addition of the linker, reacting the 4&#x2032;-OH group of the 4&#x2032;-protected-3&#x2032;-deoxypentopyranosyl nucleoside that is linked to the solid support with a 4&#x2032;-protected-3&#x2032;-deoxypentopyranosyl nucleoside phosphoramidite in the presence of a coupling reagent, and oxidizing the reaction product. This step can be repeated one or more times to produce an oligomer of desired length.

3-deoxypentopyranosyl nucleic acid, its production and its use

The invention relates to compounds including at least one pentopyranosyl nucleic acid and a biomolecule conjugated through a covalent linkage to the at least one pentopyranosyl nucleic acid. The pentopyranosyl nucleic acid has at least two pentopyranosyl nucleotide subunits covalently linked between carbon 4 and carbon 2 of their respective pentopyranosyl rings. The pentopyranosyl nucleic acid does not non-covalently bond or hybridize to naturally occurring DNA or RNA. The invention also relates to compounds comprising a biotin molecule and a pentopyranosyl nucleic acid conjugated through a covalent linkage to the biotin molecule. Processes for preparing conjugates are also described.

Pentopyranosyl nucleic acid conjugates

The invention relates to conjugates including at least one linker, a biomolecule coupled to the linker, and cyclohexane derivatives of the following formula: and oligomers thereof.

Supramolecular pairing system, its preparation and use

The invention relates to a pentopyranosylnucleoside of the formula (I) or of the formula (II) their preparation and use for the production of a therapeutic, diagnostic and/or electronic component.

Pentopyranosyl nucleic acid arrays, and uses thereof

The present invention relates to a linker nucleoside, its preparation and use for the covalent bonding of biomolecules to oligonucleotides, in particular p-RNA oligonucleotides.

Linker nucleoside, and production and use of the same

3'-Desoxypentopyranosyl nucleic acid, its production and its use

The invention relates to a compound of formula (I), wherein R is NR R , OR or SR with R and R being H or C H independently of each other and being the same or different, n being a whole number from 1 to 12; R is equal to C H &mdash;C(X)&mdash;Y with X being &boxH;O, &boxH;S or &boxH;N, Y being equal to OR , NR R or SR , R and R having the same meaning given above, and m being a whole number from 1 to 4; or R is equal to C H &mdash;Z&mdash;Y&prime; with Z being a sulfonyl, phosphonyl, ether or amine group, Y&prime; being equal to H, C H , OR , NR R or SR then Z is sulphonyl or phosphonyl group, n, R and R having the meaning given above, and Y&prime; being equal to C H when Z is an ether or an amine group; A, B, and D are the same or different and mean CR R , O, NR ou S independently of each other with R , R and R being H or C H , independently of each other, n having the meaning given above; and C is equal to CR or N with R having the meaning of R independently, A&mdash;B, B&mdash;C or C&mdash;D not being two identical heteroatoms; and nucleobase means thymine, uracile, adenine, cytosine, guanine, isocytosine, isoguanine, xanthine or hypoxanthine. The invention also relates to a method for producing these derivatives and to their use in pairing and/or testing systems.

Method for producing a pyranosyl nucleic acid conjugate

Pyranosyl nucleic acid conjugates

Method and device for revealing a catalytic activity by solid materials

The invention pertains to a process for examining chemical reactions in the presence of potentially catalytical substances, wherein reactions are triggered in miniaturized reactors with volumes of 0.001 cm to 1 cm arranged parallel to each other, and the nature and amount of the reaction mixture are analyzed during the reaction time.

Growing community of inventors

Frankfurt-am-Main, Germany

Christian Miculka