Ge Healthcare Bio-sciences Ab

Cytiva Sweden Ab

Christian Kaisermayer

Cecilia Anneren

Ann-Christin Magnusson

Mats Lundgren

Craig Robinson

Eva Lindskog

Veronique Chotteau

Rafael Diana

Johanna Tschop

Jimmie L Rucker

Therese Lundstrom

Kieron D Walsh

Gerald Blueml

The invention discloses a first connection unit having a plurality of sensor surfaces and which is adapted to be aseptically connected to a second connection unit mounted e.g. on a flexible bioreactor bag.

Aseptically connectable sensor patch

The present invention relates to a method for cell expansion. More closely, it relates to a method for expansion of cells, such as mesenchymal stem cells, on microcarriers in a plastic bag bioreactor. The invention enables expansion to therapeutic amounts of stem cells. The method comprises the following steps: a) addition of cells in cell culture medium and microcarriers to a plastic bag container; b) allowing the cells to adhere to the microcarriers while the container is kept substantially still; c) addition of further cell culture medium once the cells have adhered; d) culturing the cells under gentle and constant agitation; e) increase the surface area for continued culturing; and f) final harvesting of cells by an active detachment and separation step.

Method for cell expansion

An inflatable bioreactor bag for cell cultivation, which comprising a top and a bottom sheet of flexible material, joined together to form two end edges and two side edges, wherein one baffle or a plurality of baffles extend from the bottom sheet in a region where the shortest distance to any one of the two end edges is higher than about one fourth of the shortest distance between the two end edges.

Flexible bag for cultivation of cells

Growing community of inventors

Uppsala, Sweden

Christian Kaisermayer