Illumina Corporation

Christian Gloeckner

Kevin L Gunderson

Molly Min He

Frank J Steemers

Sasan Amini

Amirali Kia

Trina Faye Osothprarop

Jeffrey S Fisher

Erin Bomati

Ramesh Vaidyanathan

Haiying Li Grunenwald

Scott Kuersten

Darin J Haskins

Joshua Burgess

Anupama Khanna

Daniel Schlingman

Jerome J Jendrisak

Matthew William Kellinger

Lea Pickering

Kia Amirali

Embodiments provided herein relate to methods and compositions for next generation sequencing. Some embodiments include the preparation of a template library from a target nucleic acid using one-sided transposition, sequencing the template library, and capturing the contiguity information.

Methods and compositions using one-sided transposition

Presented herein are polymerase-linked nucleotides for improved distinguishing nucleotide sequences for different nucleic acid molecules. Also presented are methods and systems using the polymerase-linked nucleotides for improved distinguishing nucleotide sequences for different nucleic acid molecules.

Enzyme-linked nucleotides

Presented herein are methods and compositions for tagmentation of nucleic acids. The methods are useful for generating tagged DNA fragments that are qualitatively and quantitatively representative of the target nucleic acids in the sample from which they are generated.

Transposase compositions for reduction of insertion bias

Method of sequential tagmentation with transposase compositions for reduction of insertion bias

Embodiments provided herein relate to methods and compositions for next generation sequencing. Some embodiments include the preparation of a template library from a target nucleic acid using one-sided transposition, and sequencing the template library.

Presented herein are transposase enzymes and reaction conditions for improved fragmentation and tagging of nucleic acid samples, in particular altered transposases and reaction conditions which exhibit improved insertion sequence bias, as well as methods and kits using the same.

Modified transposases for improved insertion sequence bias and increased DNA input tolerance

Growing community of inventors

Bonn, Germany

Christian Gloeckner