Carl Zeiss Microscopy Gmbh

Carl Zeiss Microimaging Gmbh

Carl Zeiss Meditec Ag

Cad Zeiss Microscopy Gmbh

Christian Dietrich

Helmut Lippert

Benno Radt

Christopher Power

Markus Sticker

Thomas Ohrt

Wolfgang Singer

Mark Bischoff

Martin Hacker

Daniel Haase

Johannes Ruoff

Michael Kempe

Manuel Amthor

Aksel Goehnermeier

Guenter Rudolph

Lars Stoppe

Lars Omlor

Rong Dong

Timo Stich

Sebastian Backs

A method for generating an overview image of a sample which is arranged in an observation volume of a microscope by means of a sample carrier is proposed, wherein the sample carrier is illuminated by a first illumination, wherein a preliminary overview image is generated using the first illumination and an overview camera of the microscope, wherein an overview image illumination is chosen on the basis of the preliminary overview image, wherein the sample carrier is illuminated by the overview illumination, and wherein the overview image is generated using the overview image illumination and the overview camera.

Method and microscope for generating an overview image of a sample

Stereoscopy in which at least one image of a scene is recorded from a first viewing angle, and at least one image of the scene is recorded from a second viewing angle. The scene is recorded multiple times from the first viewing angle. A first combination image is obtained from the various images recorded from the first viewing angle, said combination image according to the stipulation of a comparison algorithm having smaller differences in relation to at least one image also recorded from the second viewing angle or smaller differences in relation to a second combination image obtained from the images from the second viewing angle than each individual image recorded from the first viewing angle. An image of the scene with the depth information is obtained from the first combination image and at least one image from the second viewing angle or the second combination image.

Stereoscopy method and stereoscopy apparatus

A planning device that generates control data for an ophthalmological laser therapy appliance with a laser device and a control unit, includes a first interface for supplying various data and a second interface for transferring the control data to the control unit of the ophthalmological laser therapy appliance, which is embodied to generate control data for a scanning pattern for the ophthalmological laser therapy appliance from the supplied data, by application of which scanning pattern a structure can be produced in the cornea, the limbus, and/or the sclera of a patient's eye. The planning device is embodied to generate from the supplied data, control data for the scanning pattern of this structure and/or a structure for receiving the shunt implant.

Planning device and method for generating control data for an ophthalmological laser therapy device for structures bridging the cornea in a pressure-reducing manner

Various aspects of the invention relate to techniques to facilitate autofocus techniques for a test object by means of an angle-variable illumination. Here, image datasets are captured at a multiplicity of angle-variable illumination geometries. The captured image datsets are evaluated in order to determine the Z-position.

Autofocus with an angle-variable illumination

The invention relates to a device for optical examination of a specimen with a cryo-immersion objective, having a stative, to which the cryo-immersion objective is fixed, wherein the cryo-immersion objective has a plurality of optical components, in particular lenses, and wherein the cryo-immersion objective has an optical front component which is in contact during operation with a coolable immersion liquid, having a specimen carrier for a specimen to be examined, having means for providing a cooled immersion liquid between the optical front component and the specimen to be examined on or against the specimen carrier. The device is characterized in that insulating means are present for interrupting a heat transition between the stative and the optical front component. The invention also relates to a method for examining a specimen, wherein with a device according to the invention a plurality of microscopic images are recorded, wherein for each of the individual images a different offset between a main housing and a separate housing is set. Finally the invention relates to a method for transferring a device according to the invention into an operation-ready state, wherein the components, cooled in operation, of the cryo-immersion objective, in particular the optical front component, are cooled with a coolant, in particular with liquid nitrogen, wherein the immersion liquid is cooled with a coolant, in particular with liquid nitrogen, and wherein thereafter the cooled components of the cryo-immersion objective are brought into contact with the immersion liquid.

Device for optical examination of a specimen, method for examining a specimen and method for transferring a device into an operation-ready state

A method for the three-dimensional imaging of a sample in which image information from different depth planes of the sample is stored in a spatially resolved manner, and the three-dimensional image of the sample is subsequently reconstructed from this stored image information is provided. A reference structure is applied to the illumination light, at least one fluorescing reference object is positioned next to or in the sample, images of the reference structure of the illumination light, of the reference object are recorded from at least one detection direction and evaluated. The light sheet is brought into an optimal position based on the results and image information of the reference object and of the sample from a plurality of detection directions is stored. Transformation operators are obtained on the basis of the stored image information and the reconstruction of the three-dimensional image of the is based on these transformation operators.

Method for the microscopic three-dimensional reproduction of a sample

A microscopy device, particularly for use in an imaging fluorescence lifetime microscopy method is provided. The microscopy device comprises an illumination means for generating an illumination beam, an imaging detector for spatially resolved acquisition of an emission radiation emitted by an object to be examined, an illumination beam path between the illumination means and the object to be examined, and a detection beam path between the object to be examined and the detector. The illumination beam path comprises illumination optics which are designed to generate a light sheet of illumination radiation extending transverse to the axis of the illumination beam path, wherein the axis of the detection beam path is oriented substantially perpendicular to a section plane of the light sheet and of the object to be examined. The illumination means comprise a pulsed laser.

Device for microscopy having selective illumination of a plane

A sample holding system for a microscope, including a sample chamber which has an upper opening and is filled with an immersion liquid, and in which a sample embedded in a transparent embedding compound is placed in a holder. Translatory movement of the sample in relation to a detection objective of the microscope, and rotating the sample about an essentially vertical rotational axis in a plane forming an angle different from zero with the optical axis of the detection objective is included. Rotating the sample includes a rotational drive provided with a magnetic coupling or a belt drive and/or toothed wheel rotational drive arranged above the sample chamber.

Sample holding system for a microscope with magnetic coupling

The invention is directed to an optical arrangement for photomanipulation of a sample comprising a sample holder for receiving the sample, an illumination device comprising an illumination light source and an illumination beam path for illuminating the sample with a light sheet. It further comprises a detection device for detecting light that is radiated from the sample and imaging optics which image the sample on the detection device by means of an imaging objective in an imaging beam path, wherein the light sheet is substantially planar in the focus of the imaging objective, and wherein the imaging objective has an optical axis which intersects the plane of the light sheet at an angle different from zero. Further, the arrangement also has means for photomanipulation of the sample.

Optical arrangement for photomanipulation

The invention is directed to a sample holder for a microscope. The sample holder comprises a sample chamber which is filled with an immersion liquid and in which a sample is located. The sample chamber has an upper opening. It further comprises means for translating the sample relative to a detection objective of the microscope, and means for rotating the sample around an axis of rotation extending in a substantially horizontal plane which encloses an angle other than zero degrees with the optical axis of the detection objective. In a sample holder of this kind, the sample is embedded in a transparent embedding medium having at least partially a greater solidity than the immersion liquid. Further, the sample chamber has means for horizontally supporting the embedded sample against the effect of gravity.

Sample holder for a microscope

The invention relates to methods for positioning at least one preferably biological specimen in the specimen space of a microscope arrangement, and to devices for carrying out these methods. Methods and devices are proposed, wherein the specimen's orientation relative to a detection objective's optical axis can be repeatedly changed and, in doing so, the specimen is held so that a substantially unobstructed view of the specimen is ensured from every detection direction. In different constructional variants, the specimen is held at a supporting device by adhesive forces or by a flowing medium, the specimen is held at a capillary opening by capillary action, or at least one specimen is embedded in a body of transparent gel, and the gel body is fixed in the specimen space by means of a rotatable holding device, and the detection direction is changed by rotating the holding device by a given angle of rotation.

Method for positioning biological samples in a microscopic arrangement

The invention relates to an objective replacement device for a microscope, wherein the sample is located in a sample chamber and surrounded by an immersion medium within the sample chamber, means for positioning and aligning the sample relative to the focus of an objective being present, wherein the detection beam path is aligned horizontally, which is to say perpendicular to the direction of action of gravity. For an objective replacement device for a microscope of the type described above, according to the invention a device is provided for exchanging the objective, at the focus of which the sample is positioned and aligned, with at least another objective, the position and alignment of the sample within the sample chamber remaining the same.

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Jena, Germany

Christian Dietrich