Henkel Ag & Company, Kgaa

Basf Se Corporation

Christian Degering

Nina Mussmann

Susanne Wieland

Inken Prueser

Thorsten Eggert

Daniela Herbst

Karl-Heinz Maurer

Stefan Evers

Timothy O'Connell

Allan Svendsen

Thomas Weber

Thorsten Bastigkeit

Sabine Griemert

Layla Fernandez

Christian Leggewie

Johannes Bongaerts

Daria Strauss

Shohana Islam

Sohel Dalal

Rune Nygaard Monrad

Lars Anderson

Rajendra Kulothungan Sainathan

Patrick Lorenz

Ulrich Schwaneberg

Vasudeva Prahlada Rao

Mette Louise Dissing Overgaard

Geetha Hiremath Mendez

Santhosh Vasu Mepadam

Claudia Lindner

Michael Seefried

Klaus Dorra

Britta Strauss

Felix Jakob

Ruth Schwerdtfeger

Michael Puls

Barbara Peiffer

Thomas Haarmann

Wolfgang Zimmermann

Ren Wei

Jasmin Eidner

Kristin Ruebsam

Lina Apitius

Inken Prüser

Timothy O'connell

Martina Schreiter

The present disclosure relates to a textile detergent having at least one protease and a detergent ingredient for cleaning textiles by removing protease-sensitive stains. The protease may have proteolytic activity and include an amino acid sequence having at least 70% sequence identity with the amino acid sequence given in SEQ ID NO:1 over its entire length and, in each case based on the numbering according to SEQ ID NO:1. The protease may have amino acid substitutions at the positions corresponding to positions 3, 4, 99, or 199; and at least one additional amino acid substitution at at least one of the positions corresponding to positions 74, 136, 143, 154, 161, 163, 171, 200, 203, 209, 212 or 256. The textile detergent may have a pH ranging from approximately 9 to approximately 12, measured in a 1 wt. % solution in deionized water at 20° C.

Highly alkaline textile detergent comprising protease

A washing or cleaning agent may include at least one amylase and at least one protease with an amino acid sequence having at least 70% sequence identity to the amino acid sequence specified in SEQ ID NO:1 over the entire length thereof and has an amino acid substitution at at least one of the positions corresponding to the positions 12, 43, 122, 127, 154, 156, 160, 211, 212, or 222, in each case based on the numbering according to SEQ ID NO:1. Corresponding cleaning methods and uses of such a washing or cleaning agent for cleaning textiles and/or hard surfaces are also disclosed.

Washing and cleaning agents comprising protease and amylase

Proteases may include an amino acid sequence having at least 70% sequence identity with the amino acid sequence given in SEQ ID NO:1 over its entire length. The proteases may further have, in each case based on the numbering according to SEQ ID NO:1, amino acid substitutions 9T, 130D/V, 133A, 144K, 217M, 252T and 271E at the positions corresponding to positions 9, 130, 133, 144, 217, 252 and 271. The proteases may further include at least one further amino acid substitution at at least one of the positions corresponding to positions 6, 89, 131, 166, 189, 211 or 224, and to the production and use thereof. Such proteases exhibit very good stability with good cleaning performance.

Stability-enhanced protease variants VI

Proteases may include an amino acid sequence having at least 70% sequence identity with the amino acid sequence given in SEQ ID NO: 1 over its entire length and, in each case based on the numbering according to SEQ ID NO: 1, may include (i) amino acid substitutions, such as selected from amino acid substitutions 9T, 144K, 252T and 271E, at positions corresponding to positions 9, 144, 252 and 271, and (ii) at least one additional amino acid substitution at least at one of the positions corresponding to positions 53, 120, 131, 149, 159, 162, 166, 172, 189, 192, 211, 215, 217, 224 and 274. Production and use of said proteases are described herein. Proteases of this kind demonstrate very good stability with a good cleaning performance.

Proteases having improved enzyme stability in washing and cleaning agents III

An agent, such as a detergent or cleaning agent, may include a polyesterase. A method for cleaning textiles may include applying the agent to a textile for removing soiling. The polyesterase may also help to reduce pilling effects in the agent.

Agent containing a recombinant polyesterase

Proteases may include an amino acid sequence which has at least 70% sequence identity with the amino acid sequence given in SEQ ID NO:1 over its entire length and has (i) one or more first amino acid substitutions at positions corresponding to positions 3, 4, 99, 199, or combinations thereof, and (ii) one or more second amino acid substitutions at positions corresponding to positions 9, 21, 42, 44, 105, 112, 113, 131, 137, 139, 141, 145, 159, 168, 176, 177, 182, 193, 198, 204, 205, 206, 210, 212, 230, 234, 250, 253, 255, 259, 267, or combinations thereof based on the numbering according to SEQ ID NO:1, and relates to the preparation and use thereof. Proteases of this kind demonstrate very good stability with good cleaning performance.

Performance-enhanced and storage stable protease variants

An amino acid sequence may have at least 70% sequence identity to the amino acid sequence identified in SEQ ID No. 1 over its entire length, and (a) an amino acid substitution on the position corresponding to the position 271, and (b1) at least one other amino acid substitution for at least one of the positions corresponding to the positions 18, 61, 92, 99, 137, 149, 156, 159, 162, 166, 172, 192, 199, 217, 265, or combinations thereof; and/or (b2) an amino acid substitution on the position corresponding to the position 9, and another amino acid substitution for at least one position corresponding to the positions 29, 48, 101, 130, 31, 133, 144, 217, 224, 252, or combinations thereof. Such proteases are useful for in washing or cleaning agents.

Performance-enhanced protease variants I

Detergent compositions may include endoglucanase variants and methods of using such detergent compositions. The endoglucanase may have an alteration at one or more positions where the variant has at least 60% but less than 100% sequence identity to SEQ ID NO: 2.

Detergent compositions comprising GH9 endoglucanase variants II

The present disclosure relates to proteases having an amino acid sequence with at least 70% sequence identity to the amino acid sequence given in SEQ ID No. 1, across its whole length, and comprising an amino acid substitution on at least one of the positions P9, Q10, Q62, L82, P86, N130, T141, N187, S236 or T253, relating in each case to the numbering according to SEQ ID No. 1. The present disclosure also relates to the production and use thereof. Said type of proteases have a very good cleaning performance.

Proteases with improved enzyme stability in detergents

An amino acid sequence may have at least 70% sequence identity to the amino acid sequence identified in SEQ ID No. 1 over its entire length, and (a) amino acid substitutions at the positions corresponding to the positions 9 and 271 in each case based on the numbering according to SEQ ID No. 1, and (b) an amino acid substitution on at least one of the positions corresponding to the positions 29, 48, 101, 30, 131, 133, 144, 224 or 252, in each case based on the numbering according to SEQ ID No. 1. Such proteases are particularly suitable for having an improved cleaning performance.

Performance-enhanced protease variants II

Proteases may include an amino acid sequence which has at least 70% sequence identity with the amino acid sequence given in SEQ ID NO:1 over its entire length and has, based on the numbering according to SEQ ID NO:1, (i) two or more first amino acid substitutions at positions corresponding to positions 3, 4, 99, 199, or combinations thereof; and (ii) one or more second amino acid substitutions at positions corresponding to positions 74, 136, 143, 154, 161, 163, 171, 200, 203, 209, 212, 256, or combinations thereof. Proteases of this kind demonstrate very good stability with good cleaning performance.

The aim of the invention is to improve the secretion of a protein from a host cell in order to increase the product yield of protein in a fermentation process. This is achieved by an expression vector comprising a) a promoter sequence and b) a nucleic acid sequence that codes for a protein. The protein comprises a signal peptide and an additional amino acid sequence, and the signal peptide comprises an amino acid sequence that is at least 80% identical to the amino acid sequence specified in SEQ ID NO: 2, at least 80% identical to the amino acid sequence specified in SEQ ID NO: 4, at least 80% identical to the amino acid sequence specified in SEQ ID NO: 6, or the signal peptide comprises an amino acid sequence that is structurally homologous to at least one of said sequences.

Expression vectors with promoter and nucleic acid

The present disclosure relates to proteases having an amino acid sequence with at least about 70% sequence identity to the amino acid sequence given in SEQ ID NO:2, over the entire length thereof, and comprising an amino acid substitution at at least one of the positions P9, Q62, D101, N130, G166, N187, 5216, N238 or Q271, based in each case on the numbering according to SEQ ID NO:2. The present disclosure also relates to the production and use thereof. Such proteases exhibit very good stability, in particular storage stability, while at the same time having a good cleaning performance.

The aim of the invention is to improve the secretion of a protein from a host cell in order to increase the product yield of protein in a fermentation process. This is achieved by an expression vector comprising a promoter sequence and a nucleic acid sequence that codes for a protein. The protein comprises a signal peptide and an additional amino acid sequence, and the signal peptide comprises an amino acid sequence that is at least 80% identical to the amino acid sequence specified SEQ ID NO: 2, at least 80% identical to the amino acid sequence specified in SEQ ID NO: 4, at least 80% identical to the amino acid sequence specified in SEQ ID NO: 6, or the signal peptide comprises an amino acid sequence that is structurally homologous to at least one of said sequences.

Expression vectors for an improved protein secretion

Growing community of inventors

Erkrath, Germany

Christian Degering