Centre National De La Recherche Scientifique

National Institute for Agricultural Research

Metabolic Explorer

Institut National De La Recherche

Institut National Des Sciences Appliquees De Toulouse

Institut National Des Sciences Appliquees

Institut National Des Sciences

Christian Croux

Philippe Soucaille

Patricia Sarcabal

Rainer Figge

Isabelle Meynial-Salles

Ngoc-Phuong-Thao Nguyen

Benjamin Percheron

The present invention relates to a new strain ofmodified to be unable to produce hydrogen and its use for the continuous production of bulk chemicals such as lactate, 1,3-propanediol, ethanol, butanol, isobutanol, 1,3-butanediol, acetate, acetone, isopropanol, 3-hydroxy-3-methylbutyrate and isobutene at high yield.

strains unable to produce hydrogen and useful for the continuous production of chemicals and fuels

The present invention is related to a new method for replacing or deleting DNA sequences in Clostridia, with high efficiency, easy to perform and applicable at an industrial level. This method is useful to modify several genetic loci in Clostridia in a routine manner. This method is based on a replicative vector carrying at least two marker genes.

Process for chromosomal integration and DNA sequence replacement in clostridia

The present invention is related to a new method for replacing or deleting DNA sequences in, with high efficiency, easy to perform and applicable at an industrial level. This method is useful to modify several genetic loci inin a routine manner. This method is based on a replicative vector carrying at least two marker genes.

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The invention concerns a method for preparing 1,3-propanediol from a carbon-containing substance, said method comprising a step which consists in culturing a recombinant micro-organism not producing coenzyme B12 in the absence of coenzyme B12 or one of its precursors. The invention also concerns a nucleic acid coding for a glycerol dehydratase whereof the catalytic activity is independent of the presence of coenzyme B12 or one of its precursors and a nucleic acid coding for a 1,3-propanol dehydrogenase intervening in the synthesis of 1,3-propanediol. The invention further concerns recombinant vectors and host cells comprising said nucleic acids and the polypeptides coded by the latter.

Dimeric glycerol dehydratases

Method for preparing 1,3-propanediol by a recombinant micro-organism in the absence of coenzyme B12 or one of its precursors

Method for preparing 1,3-propanediol by a recombinant micro-organism in the absence of coenzyme b12 or one of its precursors

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Auzeville Tolosane, France

Christian Croux