University of California

Affycon Aps

Christian Bech Rosen

Richard Kwant

Matthew B Francis

Meera Rao

Thomas Torring

Anne Louise Bank Kodal

Kurt Vesterager Gothelf

Michael Rosholm Mortensen

Thomas TØRRING

Provided are methods for site selective conjugation of an oligonucleotide conjugate to a metal binding protein comprising a metal binding site and for site selective conjugation of a small molecule conjugation compound (SMCoC) to an antibody comprising a metal binding site, metal binding protein conjugates obtainable by said methods, and uses of said metal binding protein conjugates.

Site selective conjugation of an oligonucleotide conjugate or a small molecule to a metal binding protein

Methods for preparing a protein conjugate having a defined number of conjugate groups are provided. The method includes: forming a mixture containing a macrocyclic matrix material and a plurality of proteins; eluting the proteins to obtain a first separated protein fraction and a second separated protein fraction, wherein substantially all of the proteins in the first separated protein fraction have the same number of handle moieties; contacting the handle moieties with a conversion reagent under conditions sufficient to convert the handle moieties in the first separated protein fraction to reactive moieties; and contacting the reactive moieties with a conjugation reagent under conditions sufficient to form a plurality of protein conjugates, wherein substantially all of the protein conjugates in the plurality have the same number of conjugate groups. Methods also include recovering enzymes and other proteins from mixtures for isolation and/or reuse of the enzymes and proteins.

Affinity-assisted protein modification and recycling

Growing community of inventors

Berkeley, CA, United States of America

Christian Bech Rosen