Tufts University

University of Pavia

Universitá Degli Studi Di Pavia

Cellink Bioprinting Ab

Christian Andrea Di Buduo

David L Kaplan

Erik Gatenholm

Lindsay Wray

Hector Martinez

Lorenzo Tozzi

Alessandra Balduini

Volodymyr Kuzmenko

Pierre-Alexandre Laurent

Itedale Redwan-namro

The present invention provides, among other things, methods for producing platelets including the steps of providing a silk membrane about 2 &#x3bc;m and 100 &#x3bc;m thick, inclusive, contacting the silk membrane with a porogen to form a porous silk membrane comprising at least one silk wall defining a lumen, associating the porous silk membrane with stromal derived factor-1? and at least one functionalizing agent, forming a three dimensional silk matrix comprising interconnected pores wherein the pores have a diameter of between about 5 and 500 &#x3bc;m, inclusive, wherein the silk matrix is formed around at least a portion of the porous silk membrane, introducing a plurality of megakaryocytes to the silk matrix such that the megakaryocytes are located at least partially within the porous silk matrix, and stimulating the plurality of megakaryocytes to produce platelets. Also provided are various new compositions and methods of making those compositions.

Microphysiologic methods and compositions

Growing community of inventors

Pavia, Italy

Christian Andrea Di Buduo