Boehringer Ingelheim International Gmbh

Christiaan Maria Reutelingsperger

Gerhard Bodo

Rudolf Hauptmann

Ingrid Maurer-Fogy

Christian Stratowa

Peter Swetly

Gunther R Adolf

Edgar Falkner

Detectably labelled annexines and compositions thereof are disclosed. Also disclosed are methods for diagnosing a disruption or activation of the hemostatic system or a prothrombotic state in an individual suspected of having a hemostatic disorder, by contacting the blood of said individual with an annexine, and detecting whether an annexine-platelet complex is formed.

Use of an anticoagulant as a diagnostic agent

This invention relates to biologically active proteins, the DNA molecules coding for them, processes for preparing them and their use.

Vascular anticoagulant proteins DNA which codes them, processer for

Pharmaceutical compositions which consist essentially of a vascular anticoagulant annexine, Ca.sup.2+, Zn.sup.2+, and a pharmaceutically acceptable carrier are disclosed. Also disclosed are processes for preparing such pharmaceutical compositions. Also disclosed are methods of preventing the coagulation of blood by mixing the blood with a vascular anticoagulant annexine, Ca.sup.2+ and Zn.sup.2+.

Composition comprising an anticoagulant

The present invention relates to processes for purifying annexines. The overall process includes the steps of preparing a homogenized cell preparation and adjusting the pH to about 8.0 to 10.0, adding at least one bivalent cation, adding a phospholipid, washing the insoluble cell residue to remove the soluble constituents, and extracting the annexines from the cell residue with a chelating agent. This process initially promotes the adsorption of the annexines onto the insoluble cell residue or membrane of the host organism. The adsorption step makes it possible to eliminate unwanted soluble matter from the homogenized material by simple washing. Desorption is accomplished by using a chelating agent.

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Maastricht, Netherlands

Christiaan Maria Reutelingsperger