Biomerieux, Inc.

Centre National De La Recherche Scientifique

Gen-Probe, Inc.

Université Joseph Fourier

Christelle Tora

Ali Laayoun

Michael McClellan Becker

Kenneth A Browne

Mitsuharu Kotera

Emmanuelle Trevisiol

Jean Lhomme

Matthew C Friedenberg

Lionel Menou

Cecile Bourget

Isabelle Sothier

Aloke R Banerjee

Fred F Hajjar

Aloke Raj Banerjee

A single- or double-stranded deoxyribonucleic acid (DNA) is fragmented and labeled by chemically fragmenting the DNA by creating at least one abasic site on the DNA, and attaching a marker to at least one of the fragments by means of a labeling reagent, the reagent covalently and predominantly coupling to at least one phosphate of the fragment. This method can be applied in the field of diagnosis.

Method for fragmenting and labeling DNA involving abasic sites and phosphate labeling

A process of fragmenting and labeling a synthetic or natural nucleic acid, comprising the steps of providing a mixture containing a nucleic acid, a labeling agent containing a detectable label, and at least one multivalent metal cation in a substantially aqueous solution; chemically fragmenting the nucleic acid in the mixture to produce a multiplicity of nucleic acid fragments; and attaching at least one label to at least one of the nucleic acid fragments to produce a detectably labeled nucleic acid fragment.

Process for labeling a nucleic acid

Growing community of inventors

Oytier Saint Oblas, France

Christelle Tora