Danisco US Inc.

Danisco US Inc., Genencor Division

Danison US Inc.

The Procter Gamble Company

Chris Leeflang

Brian E Jones

Marc Kolkman

Andrew Shaw

Melodie Estabrook

Clement K Choy

Walter Weyler

Claudine Chang

Ayrookaran J Poulose

Hiroshi Oh

Marc Anton Bernhard Kolkman

Frank Wouter Koopman

David Aaron Estell

James T Kellis, Jr

Wolfgang Aehle

Luis Gustavo Cascao-Pereira

Casper Vroemen

Ronaldus W J Hommes

Ryan L Frisch

Wilhelmus A H Van Der Kley

Leonardus P M Van Marrewijk

Scott Douglas Power

Hongxian He

Thomas P Graycar

Sandra W Ramer

Cristina Bongiorni

Steven D Doig

Casper Vroeman

Dennis De Lange

Victoria E Huang

Mansi Goyal

Eugene Steven Sadlowski

Karsten Matthias Kragh

Anita Van Kimmenade

Slavko Kralj

Wilhelmus A H Van Der Kleij

Virgil Arthur Rhodius

Leo Van Marrewijk

Marc Koklman

Leo P M Van Marrewijk

Karsten Kraugh

Stacey Irene Robida Stubbs

Johannes G De Nobel

Veli Alkan

Arjen Hoekstra

Marc Kolkmam

Brian Edward Jones

The present disclosure is generally related to compositions and methods for obtainingcells/strains having increased protein production capabilities. Certain embodiments of the disclosure are related to genetically modifiedcells/strains derived from parentalcells/strains comprising a variant rghR2 gene.

Compositions and methods for increased protein production in

The instant disclosure is generally related to compositions and methods for obtaining and constructinghost cells (e.g., protein production host cells, cell factories) having increased protein production capabilities. Certain embodiments of the disclosure are directed to efficient genetic modifications ofcells and the subsequent selection of suchcells having increased protein production capabilities. Certain other embodiments of the disclosure are generally related to methods and compositions for producing/obtaining auxotrophiccells, wherein certain other embodiments of the disclosure are directed to methods and compositions for restoring prototrophy in auxotrophiccells, and expressing genes of interest (GOIs) in such restored prototrophycells.

Methods and compositions for efficient genetic modifications ofstrains

Compositions and methods for increased protein production in bacillus licheniformis

strain WDG185 expresses an inulosucrase that efficiently synthesizes a broad range of IOS with a GF range of GF3-GF30. The isolated and/or purified inulosucrase, recombinantly engineered variants thereof, active fragments thereof, synthetic nucleic acids encoding the inulosucrase, its variants, or its active fragments, host cells comprising the synthetic nucleic acids, and compositions comprising the inulosucrase are provided. Methods of using the compositions include the manufacture of inulooligosaccharides.

Compositions and methods comprising the use of ainulosucrase (INUO)

The present disclosure is generally related to novel promoter sequences and methods thereof for enhanced protein production insp. (host) cells. As set forth herein, the novel promoter sequences of the disclosure, when operably linked to a gene or open reading frame encoding a protein of interest, are particularly well suited for use in large scale production of industrially relevant proteins.

Promoter sequences and methods thereof for enhanced protein production incells

The instant disclosure is generally related to novel engineered (hybrid) promoters. In certain embodiments, the instant disclosure is directed to one or more nucleic acid compositions comprising engineered promoters operably linked to nucleic acids encoding proteins of interest. Thus, the disclosure set forth herein described methods and compositions for the production of proteins of interest using one or more novel engineered (hybrid) promoters of the disclosure.

Engineered ribosomal promoters for protein production in microorganisms

Described are variants (mutants) of a parent alpha-amylase having alpha-amylase activity and exhibiting altered properties relative to the parent alpha-amylase, and methods of use, thereof.

TS-23 alpha-amylase variants with altered properties

Compositions and methods relating to an alpha-amylase enzyme obtained fromWDG195 are described.

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The present invention provides novel Micrococcineae spp serine proteases having multiple substitutions. In particular, the present invention provides serine proteases having multiple substitutions, DNA encoding these proteases, vectors comprising the DNA encoding the proteases, host cells transformed with the vector DNA, and enzymes produced by the host cells. The present invention also provides cleaning compositions (e.g., detergent compositions), animal feed compositions, and textile and leather processing compositions comprising these serine protease variants. In particularly preferred embodiments, the present invention provides mutant (i.e., variant) proteases derived from the wild-type proteases described herein. These variant proteases also find use in numerous applications.

Multiple mutation variants of serine protease

The present invention provides novel serine proteases, novel genetic material encoding these enzymes, and proteolytic proteins obtained fromspp., including but not limited tospp. and variant proteins developed therefrom. In particular, the present invention provides protease compositions obtained from aspp, DNA encoding the protease, vectors comprising the DNA encoding the protease, host cells transformed with the vector DNA, and an enzyme produced by the host cells. The present invention also provides cleaning compositions (e.g., detergent compositions), animal feed compositions, and textile and leather processing compositions comprising protease(s) obtained from aspp., including but not limited tospp. In alternative embodiments, the present invention provides mutant (i.e., variant) proteases derived from the wild-type proteases described herein. These mutant proteases also find use in numerous applications.

Micrococcineae serine protease polypeptides and compositions thereof

Variants ofsp. TS-23 strain alpha-amylases exhibit improved enzymatic performance, including increased thermostability, reduced calcium dependence, increased washing/cleaning performance, and baking ability. Compositions comprising these variants are useful in methods of starch processing, starch liquefaction, fermatation, starch saccharification, cleaning, laundrying, textile desizing, baking, and biofilm removal.

The present invention provides novelspp serine proteases having multiple substitutions. In particular, the present invention provides serine proteases having multiple substitutions, DNA encoding these proteases, vectors comprising the DNA encoding the proteases, host cells transformed with the vector DNA, and enzymes produced by the host cells. The present invention also provides cleaning compositions (e.g., detergent compositions), animal feed compositions, and textile and leather processing compositions comprising these serine protease variants. In particularly preferred embodiments, the present invention provides mutant (i.e., variant) proteases derived from the wild-type proteases described herein. These variant proteases also find use in numerous applications.

TS23 alpha-amylase variants with altered properties

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Leiden, Netherlands

Chris Leeflang