University of Washington

Other

Washington Research Foundation

Chiron Corporation

Research, Incorporated

Cho-chou Kuo

Guy T Mullenbach

Allan Shor

Richard Stephens

Nina Agabian

Sen-Itiroh Hakomori

Noriko Takahashi

Albertina F Swanson

J Thomas Grayston

San-pin Wang

Harlan D Caldwell

George E Kenny

Oligosaccharides specific formediate attachment of the organism to host cells.

Chlamydia oligosaccharides

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Method of diagnosing arterial chlamydial granuloma by detecting in a biological sample both a first marker associated with Chlamydia pneumoniae and a second marker associated with arterial granuloma. Therapeutic composition for treating arterial chlamydial granulomatous disease, including an anti-Chlamydia pneumoniae agent and a granuloma inhibitor.

Diagnosis and treatment of arterial chlamydial granuloma

Methods and compositions are provided for the production of a polypeptide which is immunologically cross-reactive with a naturally-occurring major outer membrane protein (MOMP) of Chlamydia trachomatis. A DNA construct including a replication system recognized by E. coli, and an MOMP gene under the transcriptional control of a .beta.-glactosidase promoter and terminator is provided. Recombinant phage .lambda.gtll/L2/33 was deposited at the American Type Culture Collection, 12301 Parklawn Drive, Rockville, Md. 20852, on Jan. 10, 1985 and granted accession no. 40157. L2 B9-F DNA was deposited at the American Type Culture Collection on Dec. 31, 1985, and granted accession No. 40217.

Immunoassays for detecting chlamydial antigens or antibodies thereto

Compositions and methods for the treatment of arterial chlamydial

Methods and compositions are provided for the production of a polypeptide which is immunologically cross-reactive with a naturally-occurring major outer membrane protein (MOMP) of Chlamydia trachomatis. A DNA construct including a replication system recognized by E. coli, and an MOMP gene under the transcriptional control of a .beta.-galactosidase promoter and terminator is provided. Recombinant phage .lambda.gt11/L2/33 was deposited at the American Type Culture Collection, 12301 Parklawn Drive, Rockville, Md. 20852, on Jan. 10, 1985 and granted accession no. 40157. L2 B9-F DNA was deposited at the American Type Culture Collection on Dec. 31, 1985, and granted accession No. 40217.

Chlamydia major outer membrane protein

Method of screening for arterial chlamydial granuloma

Methods for detecting a unique strain of chlamydia associated with acute respiratory disease are disclosed. These methods utilize monoclonal antibody directed against an antigenic determinant of the TWAR strain of chlamydia. Also disclosed is a method for determining the presence of antibodies to the TWAR strain, utilizing elementary bodies of the TWAR strain as antigen.

Detection of unique chlamydia strain associated with acute respiratory

Solubilized antigens of C. trachomatis strain LGV-434 upon analysis using two-dimensional immunoelectrophoresis yielded a single antigen which was found to be consistently precipitated by sera of patients with C. trachomatis infections. This antigen as antigen-antibody complex was employed as an immunogen to prepare a rabbit monospecific antiserum to this component or antigen. This monospecific antiserum demonstrated the presence of the antigen in each of the 15 strains of C. trachomatis organisms and was non-reactive with strains of C. psittaci. The C. trachomatis specific antigen was purified by immunoadsorption chromatography using monospecific antiserum as a specific ligand covalently bound to agarose gel columns and the resulting purified antigen employed to detect antibody from the sera of lymphogranuloma venereum patients using counterimmunoelectrophoresis. When the C. trachomatis specific antigen is isotopically labeled and utilized in the highly sensitive radioimmune assay antibody to the antigen should be demonstrated and a serological test based thereon should be applicable for the serological diagnosis of non-gonococcal urethritis (NGU).

Antigen for trachoma lymphogranuloma venereum (LGV) and non-gonococcal

Growing community of inventors

Seattle, WA, United States of America

Cho-chou Kuo