Sekisui Medical Co., Ltd.

School Juridical Person Higashi-nippon-gakuen

Chizuru Morikawa

Masahiro Ieko

Mitsuaki Yamamoto

Hirokazu Yago

Keiko Hattori

Remi Nakamura

Remi Ikeda

Hiroyuki Sunaga

Provided is the development of a convenient LA detection method in which even a sample derived from a patient who receives anticoagulant therapy of warfarin, heparin or the like, is not affected by the anticoagulant therapy, discrimination from the deficiency of blood coagulation factors is enabled, and healthy person's plasma is not used. The method for detecting lupus anticoagulant includes the following steps (A), (B) and (C): (A) a step of adding a buffer solution composition containing blood coagulation factors to each of a blood sample and a diluted sample of the blood sample before measurement or at the time of measurement of the blood coagulation time; (B) a step of measuring the blood coagulation times for the various samples of step (A); and (C) a step of comparing the blood coagulation times for the various samples obtained in step (B).

Method for detecting lupus anticoagulants

Provided is a method of measuring blood coagulation time, the method being capable of LA detection easily and with high sensitivity as compared with the method recommended by the ISTH, without being affected by deficiency of blood coagulation factors even in a blood sample of a warfarin taker, a person who suffers from vitamin K deficiency, or a hepatic failure patient. Disclosed is a method of measuring the blood coagulation time to detect lupus anticoagulant, the method including adding a buffer solution composition containing blood coagulation factors to a blood sample before measurement or at the time of measurement of the blood coagulation time, and measuring the blood coagulation time.

Method of measuring blood coagulation time to detect lupus anticoagulants

A method for measuring blood coagulation, including: preparing a reaction solution including a plasma specimen, a blood coagulation activator, and at least one of amino-guanidine and an acid addition salt thereof; and measuring blood coagulation time by detecting a turbidity change in the reaction solution.

Method for measuring blood coagulation

To provide a method for stabilizing unstable &#x3b1;-thrombin in a thrombin-containing solution, a solution containing stabilized &#x3b1;-thrombin, and a liquid fibrinogen assay reagent containing the solution. The method for stabilizing &#x3b1;-thrombin in a thrombin-containing solution, which includes adjusting the percentage of &#x3b1;-thrombin to 70% or more with respect to the amount of total thrombin in the thrombin-containing solution.

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Tokyo, Japan

Chizuru Morikawa