3M Innovative Properties Company

Chirstine A Binsfeld

Patrick A Mach

Adam J Stanenas

Mara S Celt

A composition is provided for detecting amicroorganism in sample. The composition comprises at least one first selective agent that inhibits the growth of Gram-positive microorganisms, a first differential indicator system comprising at least one first differential indicator compound that is converted to a first detectable product by amicroorganism, and a second differential indicator system comprising a second differential indicator compound that is converted by urease enzyme activity to a second detectable product. Optionally, the composition may comprise a third differential indicator system comprising a third differential indicator compound that is converted by a &#x3b2;-galactosidase enzyme activity to a third detectable product. Methods of using the composition to detect amicroorganism are also provided.

Method of detecting a salmonella microorganism

A method of detecting amicroorganism is provided. The method includes the use of a selective growth medium, a first indicator system that is converted to a first detectable product by amicroorganism, and a second indicator system that is converted to a second detectable product by &#x3b2;-galactosidase enzyme activity. The method further comprises inoculating the growth medium and incubating the inoculated growth medium at a temperature higher than 40 degrees C.

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Woodbury, MN, United States of America

Chirstine A Binsfeld