Life Technologies Corporation

Biotium, Inc.

Molecular Probes, Inc.

Invitrogen Corporation

Ching-Ying Cheung

Stephen T Yue

Wai-Yee Leung

Fei Mao

Hye Eun Hoover

David M Hagen

Jolene Ann Bradford

Jason Alfred Dallwig

Shih-Jung Huang

Gerald Thomas

Patrick Pinson

Lori Michelle Roberts

Rosaria Haugland

Diane Gray

Richard P Haugland

Wayne Forrest Patton

Thomas H Steinberg

Jie Yang

Patrick R Pinson

The present invention provides methods, compositions, and kits useful in preparing labeled molecules, which are useful in the detection of binding partners.

Fluorescent dyes, fluorescent dye kits, and methods of preparing labeled molecules

The present invention relates to fluorescent dyes in general. The present invention provides a wide range of fluorescent dyes and kits containing the same, which are applicable for labeling a variety of biomolecules, cells and microorganisms. The present invention also provides various methods of using the fluorescent dyes for research and development, forensic identification, environmental studies, diagnosis, prognosis, and/or treatment of disease conditions.

Heterocycle-substituted xanthene dyes

Cyanine dye compounds having a substituted methine moiety that are nucleic acid stains, particularly for fluorescent staining of RNA, including compounds having the formula where Ris a C-Calkyl, sulfoalkyl, carboxyalkyl or C-Calkoxy; each Ris independently selected from the group consisting of H, C-Calkyl, C-Calkoxy, fused benzo, trifluoromethyl, amino, sulfo, carboxy and halogen, that is optionally further substituted; at least one of R, R, and Ris an alkyl, aryl, heteroaryl, cyclic, or heterocyclic moiety that is optionally substituted by alkyl, amino, aminoalkyl, carboxy, nitro, or halogen; and the remaining R, Ror Rare hydrogen; X is S, O, or Se; and D is a substituted or unsubstituted pyridinium, quinolinium or benzazolium moiety.

Methine-substituted cyanine dye compounds

The present invention relates to fluorescent dyes. The present invention provides a wide range of fluorescent dyes and kits containing the same, which are applicable for labeling a variety of biomolecules, cells and microorganisms. In one aspect, the invention provides a compound having a maximal fluorescence excitation wavelength, wherein the compound has a structure of Formula II: wherein Zis a counterion, Y is a bridge unit permitting electron delocalization between F and &#x3a8;, and F is a moiety having the structure:  The present invention also provides various methods of using the fluorescent dyes for research and development, forensic identification, environmental studies, diagnosis, prognosis, and/or treatment of disease conditions.

Fluorescent compounds

The invention discloses new substituted anthraquinone dyes that may be useful as cellular stains. In some aspect of the invention, the nuclear stains are useful for staining the nuclei of dead or fixed cells. Another aspect of the invention relates to substituted anthraquinone dyes comprising an enzyme substrate moiety that is transformable or cleavable by an enzyme such that the transformation or cleavage of the substrate moiety causes a detectable change in the functionality or spectral properties of the dye.

Substituted anthraquinone dyes for cellular stains and enzyme detection

Chemically reactive carbocyanine dyes incorporating an indolium ring moiety that is substituted at the 3-position by a reactive group or by a conjugated substance, and their uses, are described. Conjugation through this position results in spectral properties that are uniformly superior to those of conjugates of spectrally similar dyes wherein attachment is at a different position. The invention includes derivative compounds having one or more benzo nitrogens.

Modified carbocyanine dyes and their conjugates

Chemical compounds having a high selectivity for double stranded DNA over RNA and single stranded DNA are disclosed. The chemical compounds are stains that become fluorescent upon illumination and interaction with double stranded DNA, but exhibit reduced or no fluorescence in the absence of double stranded DNA. The compounds can be used in a variety of biological applications to qualitatively or quantitatively assay DNA, even in the presence of RNA.

Fluorescent chemical compounds having high selectivity for double stranded DNA, and methods for their use

Luminescent metal complexes, methods of producing and/or designing same, methods of using same, and associated technology, are disclosed herein. A luminescent metal complex may be useful for a variety of applications, such as staining, detection, and/or identification, for example, of substances, such as poly(amino acids), for example. Further by way of example, a luminescent metal complex may be useful for staining, detecting, and/or identifying poly(amino acids) that are associated with any of various environments, such as a gel or a gel matrix, such as any associated with SDS-PAGE, for example, a surface environment, such as any associated with western blot, for example, and/or the like. Compositions, solutions, and kits comprising a luminescent metal complex are also disclosed herein.

Luminescent metal complexes and associated technology

The present invention provides methods and non-fluorescent carbocyanine quencher compounds having the general formula: Wherein the A moiety is a substituted pyridinium, unsubstituted pyridinium, substituted quinolinium, unsubstituted quinolinium, substituted benzazolium, unsubstituted benzazolium, substituted indolinium, or substituted indolinium. The invention further provides luminescent donor molecule-quencher pairs and luminescent donor molecule-quencher-luminescent acceptor molecule conjugates wherein the quencher is a cyanine compound of the present invention. The energy transfer pairs are used to detect an analyte of interest in a sample.

Cyanine compounds and their application as quenching compounds

The present invention relates to fluorescent dyes. The present invention provides a wide range of fluorescent dyes and kits containing the same, which are applicable for labeling a variety of biomolecules, cells and microorganisms. In one aspect, the invention provides a compound having a maximal fluorescence excitation wavelength, wherein the compound has a structure of Formula II:F&#x2014;Y=&#x3a8;&#x2003;&#x2003;Formula IIand wherein Z&#x2014; is a counterion, Y is a bridge unit permitting electron delocalization between F and &#x3a8;, and F is a moiety having the structure:  The present invention also provides various methods of using the fluorescent dyes for research and development, forensic identification, environmental studies, diagnosis, prognosis, and/or treatment of disease conditions.

Growing community of inventors

San Ramon, CA, United States of America

Ching-Ying Cheung