Taipei Medical University

Chin-Chean Wong

Shih-Ching Chen

Wen-Fu Lai

Yu-Hui Tsai

Li-Hsuan Chiu

The invention relates to an injectable hydrogel composition of 10 wt % gelatin, 0.5-2 wt % hyaluronic acid, 0.5-1.0 wt % genipin and an aqueous medium. The composition exhibits high biocompatibility, injectability and in-situ gelation ability at bone defect sites. The composition is suitable for serving as an osteogenic scaffold for cell adhesion and as an osteogenic carrier for delivering beneficial substance for promoting bone regeneration. The invention also relates to a medical formulation comprising the injectable hydrogel composition in combination with an osteogenic substance, as well as the medical use thereof in the treatment of segmental bone defect.

Injectable hydrogel for treatment of segmental bone defect

The present invention provides a method for restoring native chondrocyte phenotype and functions of, and/or increasing type II collagen as well as aggrecan mRNA expression levels and GAG accumulation level in dedifferentiated chondrocytes which have been subcultured and expanded in vitro, which comprising culturing the said dedifferentiated chondrocytes in vitro with a medium comprising type II collagen, or its biologically active peptide fragment(s) or analogs with or without growth factor(s), wherein the type II collagen or its biologically active peptide fragment(s) or analogs are effective to restore chondrocyte phenotype and functions of, and/or to increase type II collagen and aggrecan expression levels and GAG accumulation level in the said dedifferentiated chondrocytes.

Growing community of inventors

Taipei, Taiwan

Chin-Chean Wong