Sysmex Corporation

National Institute of Advanced Industrial Science and Technology

Nagoya University

National Center for Global Health and Medicine

Nagoya City University

Chikayuki Tsuruno

Shinya Nagai

Takuya Kyoutou

Hisashi Narimatsu

Yuzuru Ikehara

Atsushi Kuno

Maki Sogabe

Yasuhito Tanaka

Masashi Mizokami

Youichi Takahama

Minako Terao

Chika Mizuta

Kiyoaki Ito

Shunsuke Matsubara

Takashi Kagawa

Tadashi Ishimoto

Disclosed is a method for detecting a target substance, comprising: forming on a carrier a complex comprisinglectin (WFA) and a target substance by mixing the WFA immobilized on the carrier with the target substance comprising a sugar chain that binds to the WFA in the presence of an alcohol having 1 to 7 carbon atoms consisting of carbon atoms, hydrogen atoms, and oxygen atoms; and detecting the target substance by detecting the complex.

Method for detecting target substance, reagent for detecting target substance

The present invention relates to a method for detecting a target substance that is useful for detection of a glycoprotein and the like comprising a specific sugar chain, a reagent for detecting a target substance, a carrier and a method for manufacturing the carrier.

Reagent for detecting target substance containing sugar chain, detection method, carrier used in detection of target substance containing sugar chain, and method for manufacturing said carrier

An object of the present invention is to provide a method for measuring a glycan-marker glycoprotein, by which liver disease can be detected with higher accuracy than is possible with conventional methods. Also, an object of the present invention is to provide a method for examining liver disease, by which liver disease can be detected with higher accuracy than is possible with conventional methods. Disclosed is a method for measuring at least one glycoprotein selected from alpha-1-acid glycoprotein (AGP) and Mac-2-binding protein (M2BP) contained in a sample collected from a subject, comprising: measuring AGP binding to a first lectin selected from AOL and MAL, when the glycoprotein is AGP; and measuring M2BP binding to a second lectin selected from WFA, BPL, AAL, RCA120, and TJAII, when the glycoprotein is M2BP.

Method for measuring glycoprotein, method for examining liver disease, reagent for quantitative determination of glycoprotein, and glycan-marker glycoprotein as an index for clinical conditions of liver disease

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Hyogo, Japan

Chikayuki Tsuruno