Hokkaido Sugar Co., Ltd.

Agency of Industrial Science and Technology

Ministry of International Trade & Industry

Tax Administration Agency, Ministry of Finance

Chikashi Izumi

Masaru Yamada

Yoshimasa Takahara

Yoshiyuki Takasaki

Masahiko Nishiguchi

Kazuo Saito

Kiyoshi Yoshizawa

Shunichi Sato

Kazuhito Moriya

Shigeyoshi Narita

Tsutomu Furuya

Atsushi Mori

Makoto Tadenuma

Hitoshi Shimoi

Atsuhi Mori

Hiroshi Ishikawa

A yeast strain with high power to produce alcohol by fermentation is produced by a method comprising directly conjugating the haploid of the strain No. 909-1 with each tetrad of the ascospores of the strain No. 180 by spore-to-cell mating, acclimating the resultant zygote in the medium of waste after alcohol fermentation, and acclimating the surviving cells of the zygote in the medium of the beet molasses containing 2-deoxyglucose. The yeast strain produced is designated as Strain M-9 (FERM BP 1481) of genus Saccharomyces cerevisiae. Alcohol is produced by culturing the Strain M-9 in a medium of beet molasses.

Yeast strain with high power to produce alcohol by fermentation

A liquid starch solution suitable for saccharification into a maltose-containing product is obtained by suspending starch in water, adding to the starch suspension a heat resistant .alpha.-amylase and a carbonate, adjusting the resultant mixture to a pH value in the range of from 7.5 to 8.0, and thermally treating the resultant starch milk while repressing possible hydrolysis of starch molecules.

Process for liquefaction of starch

A liquid starch solution suitable for saccharification into a maltose-containing product is obtained by suspending starch in water, adding to the starch suspension a heat resistant .alpha.-amylase and a buffer, adjusting the resultant mixture to a pH value in the range of from 7.5 to 8.0, and thermally treating the resultant starch milk while repressing possible hydrolysis of starch molecules.

A method for the recovery of refined .alpha.-galactosidase is disclosed. This method comprises the steps of bringing an .alpha.-galactosidase-containing liquid into contact with a weakly acidic cation-exchange resin in the presence of a buffer solution for thereby allowing .alpha.-galactosidase to be selectively adsorbed by said resin, desorbing the adsorbed .alpha.-galactosidase from said resin by use of a buffer solution of a type such that at least one of the two factors, concentration and pH, has a higher value than that of the buffer solution used in said adsorption and thereafter recovering the desorbed .alpha.-galactosidase.

Method for recovery of refined .alpha.-galactosidase

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Kitami, Japan

Chikashi Izumi