Chugai Seiyaku Kabushiki Kaisha

Hoffmann-La Roche Inc.

F. Hoffmann-La Roche Ag

Chikashi Hirashima

Shinya Takuma

Yoshinori Takagi

Reinhard Franze

Thomas Link

Yuriko Tsuda

Susumu Goto

Shohei Kishishita

Herein is reported a method for the production of an immunoglobulin comprising the following steps: a) providing a eukaryotic cell comprising a nucleic acid encoding the immunoglobulin, b) cultivating the eukaryotic cell in a cultivation medium wherein the amount of glucose available in the cultivation medium per time unit is kept constant and limited to less than 80% of the amount that could maximally be utilized by the cells in the cultivation medium per time unit, and c) recovering the immunoglobulin from the culture.

Method for the production of a glycosylated immunoglobulin

It is an object of the present invention to allow a cell to produce a protein at a high level using a medium containing an enzymatic degradation product of fish meat or a fish meat extract. A method of culturing a cell comprising starting culturing in an initial medium and feeding at least once a feed medium to the initial medium during culturing, wherein at least one of the initial medium or the feed medium contains an enzymatic degradation product of fish meat or a fish meat extract added thereto. A method of producing a protein of interest using the above culture method.

Cell culture method and utilization of the same

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Tokyo, Japan

Chikashi Hirashima