Hitachi, Ltd.

Hitachi High-tech Corporation

Hitachi-high-technologies Corporation

Nippon Steel Sumitomo Metal Corporation

Chihiro Uematsu

Hideki Kambara

Kazunori Okano

Muneo Maeshima

Akira Masuya

Takashi Anazawa

Takashi Irie

Kenji Yasuda

Takahide Yokoi

Maiko Tanabe

Tomoharu Kajiyama

Tatsuro Honda

Hiroko Fujita

Hiroko Matsunaga

Hideyuki Noda

Tatsuo Nakagawa

Kyoko Imai

Masataka Shirai

Naoki Tajima

Yusuke Goto

Yoshiaki Yazawa

Makiko Takahashi

Masahiro Okanojo

Tsuyoshi Sonehara

Yoshinobu Kohara

Yutaka Wakasu

Junko Tanaka

Yuzuru Shimazaki

Yoichi Inoue

Yuichi Uchiho

Yasunori Shoji

Yasuhiro Mizuno

Kiyotaka Sugiyama

Hiroko Tada

Mima Ogawa

Yukie Nakashima

Toshiyuki Hatano

Chifumi Gouda

Kazunon Okano

Akiko Shiratori

Tetsushi Koide

The present disclosure provides a technique capable of manufacturing a measurement cell having a high specimen utilization ratio in the case of using a measurement cell that introduces a specimen into a surface hole. In the measurement cell manufacturing method according to the present disclosure, a measurement cell includes a channel wall protruding from the lower surface substrate toward the through-hole, and a specimen solution is introduced into a lower surface side space to introduce the specimen into the through-hole.

Measurement cell manufacturing method and measurement cell

Provided is a technique for preventing erroneous recognition of a fine particle region from a captured image of fine particles. A fine particle testing apparatus of the present disclosure includes: an imaging part capturing a first fine particle image of a well that holds a liquid containing fine particles; an image processor executing a process of generating a second fine particle image by extracting a contour of the first fine particle image, a process of performing a logical operation between the first fine particle image and the second fine particle image, a process of calculating a feature amount of the fine particles based on a result of the logical operation, and a process of determining growth of the fine particles in the well based on the calculated feature amount; and an output part outputting a result of the determination.

Testing apparatus and testing method

The invention provides a technology for promptly determining bacterial identification or an antimicrobial susceptibility testing. In the invention, first, a state where the bacteria are divided is monitored by performing microscopic observation with respect to the shape or the number of bacteria in each of wells of a culture plate for bacterial identification culture or the antimicrobial susceptibility testing. In addition, the shape, the number or the area of the bacteria are interpreted from the image obtained by the microscopic observation whether or not the bacteria proliferate at a stage from an induction phase to a logarithmic phase, and the time-dependent changes thereof are made into a graph. From the graph, it is determined whether or not the bacteria proliferate for each measurement, the determination results are displayed on the screen, and accordingly, the result of the antimicrobial susceptibility is provided every time when the measurement is performed.

Test device

A culturing device includes a microplate including a plurality of vessels, each of the vessels having a bottom surface having light transmittance and an opening at an upper portion, a lid having light transmittance, and an intermediate plate having light transmittance sandwiched between the lid and the microplate. The intermediate plate has a plurality of convex portions on a surface thereof facing the microplate and provided with a plurality of through holes corresponding to the convex portions that are disposed so that when the intermediate plate and the microplate are overlapped, each of the plurality of convex portions is inserted into each of the plurality of vessels and each of the plurality of through holes coincides with the opening of each of the plurality of vessels. The lid comes into contact with the intermediate plate so as to close the plurality of through holes provided in the intermediate plate.

Culture instrument

Provided is a test apparatus in which a test for bacterial identification or antimicrobial susceptibility can be promptly determined. A division state of bacteria is monitored by performing microscopic observation of shapes and the number of the bacteria in each of wells in a culture plate for bacterial identification culture or an antimicrobial susceptibility test, and it is determined whether or not the bacteria grow in a stage shifted from an induction phase to a logarithmic phase, with reference to an image obtained through microscopic observation. In addition, determination performed based on turbidity in the related art may be combined with determination performed based on microscopic observation in which change and the like in the shapes of the bacteria are monitored. Accordingly, it is possible to realize a highly accurate test result.

Test apparatus

Provided is an antibacterial agent-containing dried plate having no cracks on an observation surface (a part of the plate corresponding to an observation visual field of a microscope). According to the present embodiment, an antibacterial agent-introduced plate obtained by introducing an antibacterial agent and performing vacuum drying has a recess at an edge of a well, and a microscopic observation portion, which has a surface substantially parallel to a well bottom surface, near the center of the well. The recess is provided between the microscopic observation portion and a side wall of the well, and is lower in height than the microscopic observation portion. Further, at least the bottom surface of the well and the microscopic observation portion are made of a material having a light-transmitting property in order for optical measurement (FIG.).

Bacterial test plate having antibacterial agent introduced thereinto, and transparent plate

An electrophoresis method, system, and gel recover biological substances from the gel with high efficiency. The method uses an electrophoresis gel having an injection hole into which biological substances are injected and a recovery hole from which the biological substances are recovered. The electrophoresis method includes injecting the biological substances into the injection hole, and applying an electric field penetrating the injection and recovery holes. A vertical axis in a downward direction as a positive direction is set as an X-axis, an axis which is perpendicular to the X-axis is set as a Y-axis, and coordinates of a bottom of the recovery hole are set as (X, Y). The X coordinate Xof the bottom of the recovery hole satisfies X&#x3e;Xwhen the biological substance is electrophoresed to coordinates (X, Y) in the recovery hole from a bottom of the injection hole in the applying the electric field.

Electrophoresis method, electrophoresis system, and electrophoresis gel

A light detection device comprises: a flow path section in which a plurality of lines of flow of a plurality of dropletsdispersed in oil and moving along a linear line of flow is retained on an array plane; a laser beam irradiation section which introduces a laser beamin a direction in which the plurality of lines of flow are arrayed in the flow path section to irradiate the plurality of lines of flow; and a light detection section which detects emitted light generated from the plurality of lines of flow by the irradiation of the laser beam, from a vertical direction with respect to the array plane. A refractive index of the oil no and a refractive index of the droplets nd have a difference such that &#x2212;0.02&#x2264;nd&#x2212;no&#x2264;0.05.

Light detection device

The invention provides a technology for promptly determining bacterial identification or an antimicrobial susceptibility testing. In the invention, first, a state where the bacteria are divided is monitored by performing microscopic observation with respect to the shape or the number of bacteria in each of wells of a culture plate for bacterial identification culture or the antimicrobial susceptibility testing. In addition, the shape, the number or the area of the bacteria are interpreted from the image obtained by the microscopic observation whether or not the bacteria proliferate at a stage from an induction phase to a logarithmic phase, and the time-dependent changes thereof are made into a graph. From the graph, it is determined whether or not the bacteria proliferate for each measurement, the determination results are displayed on the screen, and accordingly, the result of the antimicrobial susceptibility is provided every time when the measurement is performed (FIG.).

Provided is a antimicrobial susceptibility testing device, including: an ATP examination culture plate that includes a reaction vessel, a reagent holding parts for holding reagents to be supplied to the reaction vessel, and a culture solution holding part for holding a culture solution to be supplied to the reaction vessel, and has plural layers that can be joined and separated; a gas feeding path for feeding a gas into the ATP examination culture plate; a heater; an optical detection unit; and a determination unit for determining sensitivity of a bacterial strain contained in the culture solution to a drug based on a detection result of the optical detection unit, wherein when the plural layers of the ATP examination culture plate are joined, at least the culture solution holding part and the reaction vessel are in a sealed state while communicating with each other.

Antimicrobial susceptibility testing device, antimicrobial susceptibility testing kit, and antimicrobial susceptibility testing method

A nucleic acid amplification method includes ligating a double-stranded adapter () containing adapter DNA strands capable of forming a folded structure to a double-stranded DNA () containing a target DNA sequence () to prepare a cyclic DNA template composed of double-stranded DNA containing a nick (). A 3'-end elongation reaction is performed using a strand-displacement DNA polymerase from the nick () as an origin, thereby producing a concatemer () in which a plurality of the target DNA sequences () and the adapter DNA strands capable of forming the folded structure are linked in series as a single-stranded DNA. The concatemer () contains a plurality of the target DNA sequences () suitable for nucleotide sequence analysis and has a folded shape such that it takes the form of a ball due to its folded structure.

Nucleic acid amplification method

A surface temperature measuring apparatus comprising a radiation thermometer configured to detect thermal radiation light emitted from a surface of a temperature measurement target material in a cooling process with water, a housing having an opening on a temperature measurement target material side, the housing storing, in an inside of the housing, at least a light receiving unit of the radiation thermometer among structural elements of the radiation thermometer, and an optical glass that is fit and sealed in the inside of the housing between the temperature measurement target material and the light receiving unit of the radiation thermometer, the optical glass being configured to transmit the thermal radiation light. The optical glass has, on the temperature measurement target material side, an end surface adjacent to the surface of the temperature measurement target material. A surface temperature measuring method is also provided.

Surface temperature measuring apparatus and surface temperature measuring method

This invention concerns a sample processing device capable of efficiently recovering biological molecules, such as nucleic acids or proteins. The sample processing device is capable of placing a reaction container having a plurality of reaction sites, and it comprises a nozzle mechanism with a nozzle capable of attaching and removing a dispenser tip for dispensing a solution into the reaction sites of the reaction container and a magnetic tip for generating a magnetic field that allows magnetic beads to migrate to a space among the plurality of reaction sites in the reaction container, and a drive control unit controlling the nozzle mechanism.

Sample processing device, sample processing method, and reaction container used in these device and method

Nucleic acid analysis apparatus includes a plurality of temperature adjustment apparatuses, a rotating mechanism, a delivery base and an ejection base, a delivery drive mechanism, an ejection drive mechanism, and a detection apparatus. The rotating mechanism can include a rotating shaft and a plurality of pressing portions that rotate around the rotating shaft. The reaction plate assembly can move over the temperature adjustment apparatuses along the circumferential direction in a state of being pressed onto the temperature adjustment apparatuses by the pressing portions. The delivery drive mechanism can cause the reaction plate assembly to be moved radially inward and delivered between the pressing portions and temperature adjustment apparatuses. The ejection drive mechanism can cause the reaction plate assembly to be moved radially outward and ejected from between the pressing portions and temperature adjustment apparatuses onto the ejection base. Reaction plates, reaction plate assemblies, and nucleic acid analysis apparatuses are provided.

Reaction plate assembly, reaction plate and nucleic acid analysis device

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Tokyo, Japan

Chihiro Uematsu