Sanofi

The Government of the United States of America As Represented by the Secretary of the Deptartment of Health and Human Services

Chih-Jen Wei

Gary J Nabel

Kurt Swanson

Te-Hui Chou

Laura Nguyen

Jeffrey C Boyington

Hadi M Yassine

Patrick M McTamney

Pradeep K Dhal

Masaru Kanekiyo

Ronnie Rong Wei

Magnus Besev

Masaro Kanekiyo

Stefan Koester

Ram Dharanipragada

Heather Kamp

Patrick M Mctamney

This disclosure relates to antigenic EBV polypeptides and their use in eliciting antibodies against EBV. Also disclosed are antigenic polypeptides comprising an EBV polypeptide and a ferritin protein.

Antigenic Epstein Barr virus polypeptides

Antigenic respiratory syncytial virus (RSV) polypeptides for use in eliciting antibodies against RSV are provided. Also provided are antigenic polypeptides comprising an RSV polypeptide and a ferritin protein.

Antigenic respiratory syncytial virus polypeptides

Antigenic epstein barr virus polypeptides

This disclosure relates to antigenic OspA polypeptides and their use in eliciting antibodies against OspA. Also disclosed are antigenic polypeptides comprising an OspA polypeptide and a ferritin protein.

Antigenic OspA polypeptides

Ferritin proteins comprising a mutation replacing a surface-exposed amino acid with a cysteine, an N- or C-terminal linker comprising a cysteine, and/or one or more immune-stimulatory moieties linked to the ferritin protein via a surface-exposed amino acid are disclosed. The ferritin proteins can further comprise a non-ferritin polypeptide and be antigenic, e.g., for use in eliciting antibodies against the non-ferritin polypeptide.

Ferritin proteins

Novel vaccines are provided that elicit broadly neutralizing anti-influenza antibodies. Some vaccines comprise nanoparticles that display hemagglutinin trimers from influenza virus on their surface. The nanoparticles comprise fusion proteins comprising a monomeric subunit of ferritin joined to at least a portion of an influenza hemagglutinin protein. Some portions comprise the ectodomain while some portions are limited to the stem region. The fusion proteins self-assemble to form the hemagglutinin-displaying nanoparticles. Some vaccines comprise only the stem region of an influenza hemagglutinin protein joined to a trimerization domain. Such vaccines can be used to vaccinate an individual against infection by heterologous influenza viruses and influenza virus that are antigenically divergent from the virus from which the nanoparticle hemagglutinin protein was obtained. Also provided are fusion proteins and nucleic acid molecules encoding such proteins.

Nucleic acid molecules encoding ferritin-hemagglutinin fusion proteins

Influenza hemagglutinin protein-based vaccines

Growing community of inventors

Potomac, MD, United States of America

Chih-Jen Wei