Industrial Technology Research Institute

Chih-Hsi Liu

Jo-Shu Chang

Yin-Lung Han

Chieh-Lun Cheng

Yung-Chong Lou

Tai-Rong Guo

Wan-Ju Yu

The disclosure provides a culturing medium for culturing a bacterium of genus, including: a carbon source which is an organic acid, selected from a group consisting of acetate, lactate and butyrate; a nitrogen source selected from a group consisting of ammonium sulfate ((NH)SO), ammonium nitrate (NHNO), ammonium chloride (NHCl) and urea; phosphate; magnesium chloride (MgCl); yeast extract; and trace elements.

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The disclosure provides a method for producing extracellular proteins from genus, including: performing a fermentation culturing to a bacteria of genuswith a culturing medium to form a fermented liquid, wherein the composition of the culturing medium includes a carbon source which is an organic acid, selected from a group consisting of acetate, lactate and butyrate; a nitrogen source selected from a group consisting of (NH)SO, NHNO, NHCl and urea; phosphate; carbonate; MgCl; yeast extract; and trace elements, and wherein the gas feeding rate for the fermentation culturing is about 0-0.1 vvm; and after the fermentation culturing is completed, collecting the fermented liquid, wherein the fermented liquid contains extracellular protein secreted from the bacteria of genus

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Hsinchu, Taiwan

Chih-Hsi Liu