Massachusetts Institute of Technology

Chih-Chieh Yu

Edward Stuart Boyden

Linyi Gao

Ruixuan Gao

Fei Chen

Asmamaw T Wassie

Paul Warren Tillberg

Or A Shemesh

The invention encompasses hydrogels, monomer precursors of the hydrogels, methods for the preparation thereof, and methods of use therefor. The linking of monomers can take place using non-radical, bioorthogonal reactions such as copper-free click-chemistry.

Swellable and structurally homogenous hydrogels and methods of use thereof

The invention, in some aspects relates to compositions and methods for imaging biological systems and physiological activity and conditions in cells.

Stochastic arrangement of reagents in clusters&#x2014;STARC

The invention provides a method termed protein retention ExM (proExM), in which proteins, rather than labels, are anchored to the swellable gel, using a cross-linking molecule. This proExM strategy can be used to perform nanoscale imaging of immunostained cells and tissues as well as samples expressing various FPs as fluorescent signals from genetically encoded fluorescent proteins and/or conventional fluorescently labeled secondary antibodies and streptavidin that are directly anchored to the gel are preserved even when subjected to the nonspecific proteolytic digestion.

Protein retention expansion microscopy

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Cambridge, MA, United States of America

Chih-Chieh Yu