Transgenic Inc.

Kumamoto Technology and Industry Foundation

Chiemi Minoda

Nobuo Sakaguchi

Kazuhiko Kuwahara

Masao Kawakita

Shingo Shinagawa

Kyoko Hiramatsu

Yusei Nagase

Mika Ito

Yosuke Mizukami

The present invention provides an anti-diacetylspermine specific monoclonal antibody which satisfies a specific measuring condition in an immunoreaction system between a solid-phased diacetylspermine and the anti-diacetylspermine specific monoclonal antibody, wherein the immunoreaction system comprises a sample diacetylspermine or sample N-acetylspermidine to inhibit the immunoreaction; and the specific measuring condition is that the 50% inhibition activity against the immunoreaction by the diacetylspermine in the sample is at least 1,000 times as much as the 50% inhibition activity against the immunoreaction by the N-acetylspermidine in the sample.

[object Object]

The present invention aims at providing a high affinity anti-HIV antibody. According to the present invention, there are provided an antibody or a fragment thereof that binds to the gp12 glycoprotein of HIV and has a dissociation constant (KD) value of 1.0&#xd7;10(M) or less; a pharmaceutical composition comprising the antibody or fragment thereof; and a method of producing an anti-HIV antibody or a fragment thereof, comprising immunizing a GANP transgenic non-human mammal or a progeny thereof with a polypeptide consisting of the amino acid sequence as shown in SEQ ID NO: 6 as an antigen and collecting the antibody from the resultant mammal or progeny.

Anti-HIV antibody

Growing community of inventors

Kumamoto, Japan

Chiemi Minoda