Riken Corporation

Chie Takemoto

Shigeyuki Yokoyama

Akiko Tanaka

Mikako Shirouzu

Takanori Kigawa

Namtip Chumpolkulwong

Natsuko Matsuda

It is intended to provide a process for producing an extract for cell-free protein synthesis whereby the productivity of a protein and the production efficiency can be improved. Cells are cultured under suppressed growth conditions. In the stationary phase of the culture, the cells are collected and then disrupted. The above-described cells are preferably bacterial cells, in particular,cells. In the suppressed growth conditions as described above, the culture temperature is preferably from 20 to 32&#xb0; C., more preferably 26&#xb0; C. or higher and lower than 30&#xb0; C.

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Yokohama, Japan

Chie Takemoto