Daikin Industries, Ltd.

National Institute of Advanced Industrial Science and Technology

The Jikei University School of Medicine

Chiaki Okumura

Junichiro Arai

Hideo Katayama

Sadayori Hoshina

Takashi Ushida

Yoshihisa Amano

Katsuhiko Machida

Megumi Akamatsu

Naoki Fukui

Seiichiro Miyahara

A method of measuring the number of bacteria according to the present invention includes step (a) through step (d). First, in step (a), a to-be-measured sample (specimen) including a predetermined bacterial strain (such asor coliform bacteria) is added to a predetermined medium (such as a medium used for a specific enzyme substrate culture medium method). In step (b), a current value flowing through the medium added with the sample is measured with an oxygen electrode at a predetermined temperature and at a predetermined constant voltage. In step (c), time required is measured for the current value that has decreased temporarily after starting the measurement of step (b) to increase thereafter to exceed a predetermined threshold value. In step (d), the initial number of bacteria of the bacterial strain included in the sample is calculated based on the time required.

Method of measuring the number of bacteria, device of measuring the number of bacteria and cell used in the device

Disclosed herein is a method for determining the drug sensitivity of a microbe which comprises pouring a microbial suspension into each of the compartments or wells at a position which is in the vicinity of an electrode for measuring dissolved oxygen concentration, measuring current from each electrode at a second time interval for a third time period, each time is obtained based upon each of the measured currents at which the maximum current is obtained, obtaining each current value within a fourth time period which starts from the time at which the maximum current is obtained, detecting drug sensitivity based upon the variation condition of each current value during the fourth time period. The method allows rapid drug susceptibility measurements.

Drug sensitivity measuring method

A method and apparatus wherein measurement current values output from a first oxygen sensor and a second oxygen sensor are time sequentially measured. Each moving average value is calculated from each sequential measurement current value. Each time differential value is calculated from the pair of each calculated moving average values, by least squares approximation. Then, drug susceptibility is measured based upon each calculated time differential value, so that drug the susceptibility measurement is performed quickly or accurately.

Drug susceptibility measurement method and apparatus thereof

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Ibaraki, Japan

Chiaki Okumura