Amyris, Inc.

Amyris Bio Products Portugal, Unipessoal, Lda

Chia-Hong Tsai

Andrew Main

Kevin George

Yoseph Tsegaye

Victor Holmes

Phoebe Yeh

Christopher J Paddon

Provided herein are compositions and methods of integrating one or more exogenous donor nucleic acids into one or more exogenous landing pads engineered into a host cell's genome. In certain embodiments, the exogenous landing pads and exogenous donor nucleic acids comprise standardized, compatible homology regions so that exogenous donor nucleic acids can integrate into any of the landing pads, independent of the genomic sequences surrounding the landing pads. In certain embodiments, the methods comprise contacting the host cell comprising landing pads with one or more exogenous donor nucleic acids, and a nuclease capable of causing a double-strand break within the landing pads, and recovering a host cell comprising one or more exogenous donor nucleic acids integrated in any of the landing pads.

Compositions and methods for genomic integration of nucleic acids into exogenous landing pads

Provided herein are compositions and methods for co-production and recovery of two or more isoprenoids from a single recombinant cell.

Co-production of a sesquiterpene and a carotenoid

Growing community of inventors

Martinez, CA, United States of America

Chia-Hong Tsai