Motorola Corporation

Princeton University

Chia Fu Chou

Frederic Zenhausern

Herbert Goronkin

Robert H Austin

Jonas O Tegenfeldt

Wayne Frasch

Daniel J Sadler

Lars Chapsky

Edward C Cox

Rajnish G Changrani

Olgica Bakajin

An exemplary system and method of employing DNA hybridization for the detection of bio-agents is disclosed as comprising inter alia a biomolecular rotary motor (); a capture probe DNA fragment () effectively attached to said biomolecular motor (); a target DNA fragment () suitably adapted for hybridization with said capture probe DNA (); a signal probe DNA fragment () suitably adapted for hybridization with said target DNA (); and a fluorescent bead () attached to said signal probe DNA (). Disclosed features and specifications may be variously controlled, adapted or otherwise optionally modified to improve certain device fabrication parameters and/or performance metrics.

Single molecule detection of bio-agents using the F1-ATPase biomolecular motor

A method for mixing at least two fluids includes introducing the at least two fluids into a mixing chamber. The mixing chamber includes a piezoelectric component () for mechanical actuation of fluid motion within or adjacent the mixing chamber. The piezoelectric component includes at least first (), second (), third (), and fourth () actuation domains, the first and third actuation domains being on first and third opposed sides of the piezoelectric component, and the second and fourth actuation domains being on second and fourth opposed sides of the piezoelectric component. The first and third domains are actuated at a first phase of a frequency of oscillation, and the second and fourth domains are actuated at a second phase of the frequency of oscillation.

Piezoelectric mixing method

The present invention further provides a device for the integrated micromanipulation, amplification, and analysis of polarized particles such as DNA comprises a microchip which contains constrictions of insulating material for dielectrophoresis powered by an alternating current or direct current signal generator, and attached to a hot source that can be heated to specific temperatures. Nucleic acids can be heated and cooled to allow for denaturation, and the annealing of complementary primers and enzymatic reactions, as in a thermocycling reaction. After such a reaction has been completed at the constriction, the dielectrophoretic field can be switched to a direct field to release the product and direct it through a matrix for fractionation. The device includes data analysis equipment for the control of these operations, and imaging equipment for the analysis of the products. The invention permits the efficient handling of minute samples in large numbers, since reactions occur while sample material is trapped between constrictions. Because the positioning, reactions, and release into a fractioning matrix all occur at the constriction which serves as a focusing locus, the need to transfer samples into different tubes is eliminated, thus increasing the efficiency and decreasing the possibility of damage to the samples.

Electrode-less dielectrophorises for polarizable particles

Growing community of inventors

Chandler, AZ, United States of America

Chia Fu Chou