Academia Sinica

Chi-Yao Chang

Jyh-Yih Chen

Jen-Leih Wu

Chia-Ching Chang

Chih-Tung Tsai

Kuen-Lin Leu

Jing-Wen Ting

Chih-Hung Lin

The invention relates to new fish growth hormones, nucleic acids encoding them, and transgenic fish that express them.

Fish growth hormones

The present invention relates to the finding and construction of fish insulin-like growth factor II (IGF-II) cDNAs which can be cloned and expressed in cells. This invention also relates to the production of biologically active fish IGF-II polypeptides by a gene expression system using fish IGF-II cDNAs. The fish IGF-II cDNAs have 1977 bp which transcribe into a prepeptide (signal peptide), and B, C, A, D, E domain peptides. The fish mature IGF-II is a single polypeptide containing the NH.sub.2 -B-C-A-D-COOH domains. The mature IGF-II polypeptide is 7 kDa in weight and has 70 amino acids. The fish recombinant IGF-II CDNA can be cloned and expressed in E. coli, yeast, baculovirus, and fish cells.

Production of biologically active recombinant insulin-like growth factor

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Taipei, Taiwan

Chi-Yao Chang