Wisconsin Alumni Research Foundation

National Institutes of Health, a Component of the US Dept. of Health & Human Services

Academia Sinica

Chi-Ming Liang

Donald T Witiak

Jei-Ming Peng

Carmen J Allegra

Bruce A Chabner

Patrick G Johnston

Ching-Feng Chiu

Shao-Wen Hung

Nancy A Turner

Shu-Mei Liang

Chun-Yung Huang

The present invention relates to monoclonal antibodies that are specific for the protein thymidylate synthase, and hybridomas producing these monoclonal antibodies. The invention further relates to methods of detection and diagnostic kits to test for the presence of thymidylate synthase. The invention also relates to the use of the monoclonal antibodies in determining the presence of colon carcinoma cells.

Monoclonal antibodies specific for human thymidylate synthase

A method for identifying patients at risk of developing vascular leakage syndrome and systemic inflammatory response syndrome (SIRS) such as septic shock by determining the serum levels of metalloproteinase expression, in particular type IV collagenase expression, as well as a method of treating or preventing vascular leakage syndrome and SIRS by the administration of one or more metalloproteinase inhibitors, preferably type IV collagenase inhibitors is taught. Additionally, the therapeutic efficacy of bis(dioxopiperazine) compounds is determined on the basis of collagenase inhibiting activity, and the compounds which inhibit collagenase activity are utilized for the treatment of collagenase related disorders, e.g., vascular leakage syndrome, septic shock, stroke, cardiac disorders, angiogenesis, and arthritis. Finally, a method for preventing or treating toxicity caused by endogenous cytokine expression or cytokine administration or by immunotoxin administration by the administration of one or more metalloproteinase inhibitors, preferably type IV collagenase inhibitors is taught.

Treatment of vascular leakage and related syndrome such as septic shock

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Bethesda, MD, United States of America

Chi-Ming Liang