Dr. Chip Biotechnology Incorporation

Dr. Chip Biotechnology Inc.

Chi-Horng Bair

Shin-Hwan Wang

Kan-Hung Lee

Yih-Weng Wang

Yu-Hau Shih

Hsiung Hsiao

Chuan-Mei Tsai

Yang-Yuan Tseng

This invention features a biomolecule-bound substrate that includes a support made of an organic polymer and having an unmodified surface; and a plurality of unmodified biomolecules immobilized on the unmodified surface. The organic polymer is acrylic resin, polypropylene, polystyrene, polyethylene, polyvinyl chloride, polysulfone, polycarbonate, cellulose acetate, rubber, latex, polyethylene terephthalate, acrylonitrile butadiene styrene, acrylonitrile styrene, or a combination thereof. The substrate is formed by placing the unmodified biomolecules on the unmodified surface followed by ultraviolet irradiation.

Biomolecule-bound substrates

The present invention discloses pairs of oligonucleotide primers for use in detecting the presence or absence of an enterovirus in a sample. Also disclosed are synthetic nucleotide sequences capable of specifically hybridizing to a sense strand of an enterovirus nucleic acid or a nucleic acid corresponding to the sense strand. The present invention provides a method of detecting the presence or absence of an enterovirus nucleic acid in a sample as well as a kit for detecting an enterovirus in a sample. The present invention particularly provides a method and a kit for detecting and differentiating enterovirus type 71 and/or coxsackievirus A16 in a sample.

Methods for detecting and differentiating enteroviruses and the primers and probes therefor

Growing community of inventors

Tucheng, Taiwan

Chi-Horng Bair