Ortho Diagnostic Systems Inc.

Chi-Deu Chang

Henry A Graham, Jr

Ernest G Schutt

Houston G Brooks, Jr

Utpal R Chakraborty

Jennifer A Nasser

Sharon R Lawler

Lloyd L Hollenbeck, Jr

Albert Venturini

A method and test kit useful in a wide variety of immunoassay systems. A method of producing metal sol reagents containing metal sol particles of a preselected size is provided. A metal containing solution is reduced under optimized pH conditions to produce metal sol particles of a preselected size, the particles are coated with a coupling compound and then bound with at least one selected immunochemically reactive component. Particles having different immunochemical specificities are also mixed to produce reagents having multiple selected immunochemical specificities.

Kit containing glutaraldehyde coated colloidal metal particles of a

Method of producing a metal sol reagent containing colloidal metal

Reagents and methods are described for an immunoassay test of increased sensitivity and decreased complexity employing an immunoglobulin specific for an antigen naturally or artificially placed upon the surface of an indicator particle coupled through the use of a hetero-bifunctional coupling reagent to a second antibody of differing specificity and specific for the antigen to be detected. In a preferred embodiment, a hetero-bifunctional coupling agent couples via a sulfhydryl group, a univalent immunoglobulin specific for the surface antigens on erythrocytes to a second multivalent immunoglobulin through an amide linkage with the latter immunoglobulin wherein said second immunoglobulin is specific for hepatitis-B surface antigen.

Double antibody conjugate

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Bridgewater, NJ, United States of America

Chi-Deu Chang