Lallemand Hungary Liquidity Management LLC

Stellenbosch University

Mascoma Corporation

Danstar Ferment Ag

Universiteit Stellenbosch

Chhayal V Gandhi

John E McBride

Anne K Warner

Elena E Brevnova

Erin Wiswall

Mark Mellon

Aaron Argyros

Trisha Barrett

Nicky Caiazza

Vineet Rajgarhia

Allan C Froehlich

Charles F Rice

Ryan Skinner

Emily Stonehouse

Riaan Den Haan

Shital A Tripathi

Haowen Xu

Kristen M Deleault

John S Bardsley

Frank Agbogbo

Kevin S Wenger

Indraneel Shikhare

Vineet B Rajgarhia

Abigail Foster

Jennifer Gosselin

Yin-Ying Chiu

Alan Belcher

Heidi H Hau

Heidi Lau

Jim Flatt

Elena Brevnova

John Mcbride

The present invention is directed to a yeast strain, or strains, secreting a full suite, or any subset of that full suite, of enzymes to hydrolyze corn starch, corn fiber, lignocellulose, (including enzymes that hydrolyze linkages in cellulose, hemicellulose, and between lignin and carbohydrates) and to utilize pentose sugars (xylose and arabinose). The invention is also directed to the set of proteins that are well expressed in yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze starch and cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less glycerol and uptake acetate. The resulting strain, or strains can also be used to produce ethanol from granular starch without liquefaction. The resulting strain, or strains, can be further used to reduce the amount of external enzyme needed to hydrolyze a biomass feedstock during an Simultaneous Saccharification and Fermentation (SSF) process, or to increase the yield of ethanol during SSF at current saccharolytic enzyme loadings. In addition, multiple enzymes of the present invention can be co-expressed in cells of the invention to provide synergistic digestive action on biomass feedstock. In some aspects, host cells expressing different heterologous saccharolytic enzymes can also be co-cultured together and used to produce ethanol from biomass feedstock.

Yeast expressing saccharolytic enzymes for consolidated bioprocessing using starch and cellulose

The present invention provides for heterologous expression of termite and termite-associated symbiont cellulases. The cellulases can, for example, be codon-optimized and expressed in yeast host cells, such as the yeast. The cellulases can also be co-expressed in host cells with other cellulases. The expression in such host cells of the termite and termite-associated symbiont cellulases, and variants and combinations thereof, result in yeast with improved cellulosic activity. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems.

Heterologous expression of termite cellulases in yeast

Heterologous expression of termite cellulases yeast

The present invention provides for the isolation and characterization of the cbh1 gene from. In particular, the present invention provides for the nucleic acid and amino acid sequences ofcbh1, and domains, variants and derivatives thereof. The present invention further provides for the heterologous expression ofCbh1 in host cells, including yeast, e.g.,. Expression ofCbh1 in host cells will augment cellulose digestion and facilitate ethanol production by those host cells on cellulosic substrates. In certain embodiments, heterologous expression inis in coordination with heterologous expression of other known, or newly identified saccharolytic enzymes. Therefore, the present invention also provides that the novelCbh1 gene can utilized in a consolidated bioprocessing system.

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Lebanon, NH, United States of America

Chhayal V Gandhi