Other

Agdia Inc.

Chester L Sutula

R Michael Roe

George G Kennedy

Fred L Gould

Woodward D Bailey

Murali D Bandla

Matthew R Chambers

A method for performing an immunoassay is described. The method is particularly useful for detecting extracellular polysaccharide (EPS) and/or lipopolysaccharide (LPS) producing microorganisms. The method is particularly useful for detecting microorganisms which produce extracellular polysaccharides (EPS) also known as exocellular polysaccharides, capsule, and/or lipopolysaccharides (LPS). In a preferred method for detecting microorganisms which produce EPS, LPS, or both, the EPS and/or LPS is extracted from a sample with cetyltrimethylammonium bromide (CTAB) to produce molecular aggregates which are then preferentially bound to colored polystyrene latex particles over other components in the sample, and the bound EPS and/or LPS detected using a lateral flow immunoassay apparatus which has immobilized thereon antibodies specific for the EPS and/or LPS. The method can also be used to detect particular viruses, for example viruses of the potyviridae or tobamoviridae group.

Immunoassay and method of use

Methods of assaying insects for pesticide resistance and to identify insect species are based on feeding disruption caused by insecticide such as the biopesticide toxin (Bt). Further provided are methods for monitoring insecticide resistance in a population of insects, as well as methods of screening compounds for insecticidal activity. Insecticide resistance can be assessed at the level of an individual insect or at population levels. Apparatus and dehydrated insect meal pads useful in such assays are described.

Insecticide resistance assay

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Elkhart, IN, United States of America

Chester L Sutula