Pacific Biosciences of California, Inc.

Genapsys, Inc.

Applied Biosystems, LLC

The UAB Research Foundation (The University of Alabama at Birmingham)

Cheryl Heiner

Kevin Travers

Stephen Wilson Turner

Mark F Oldham

Eric S Nordman

Richard T Reel

Hesaam Esfandyarpour

Frank Lee

Geoff Otto

Kosar Baghbani Parizi

Congcong Ma

Susanne Baumhueter

Jonas Korlach

Jon M Sorenson

Tyson A Clark

Erik Miller

Benjamin A Flusberg

Jeremiah Hanes

Patrick Marks

Austin B Tomaney

Dale R Webster

Andrey Kislyuk

Pranav Patel

Keith P Bjornson

John Lyle

Jeffrey Wegener

Lei Jia

Shiaw-Min Chen

Igor D Vilfan

Timothy Hunkapiller

Jessica Lee

Curtis Gehman

James Labrenz

John S Eid

John Irvin Glass

Kenneth Mark Maxham

Joseph Puglisi

Elliot Lefkowitz

Jennifer S Glass

Ellson Y Chen

Gail H Cassell

Kosar Baghbani Parazi

Methods, compositions, and systems are provided that allow for reliable sequencing of the initial sequence region of a sequence of interest. The methods of the invention allow for more reliable barcoding of subpopulations of nucleic acids to be sequenced.

Delaying real-time sequencing

Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.

Classification of nucleic acid templates

Methods and systems for single molecule sequencing using concatemers of copies of sense and antisense strands. Concatemers are provided, for example, by carrying out rolling circle amplification on a circular molecule having sense and antisense regions to produce repeated copies of the sense and antisense regions connected by linking regions. The circular molecules can be produced by ligating hairpin adapters to each end of a double-stranded nucleic acid having a sense and antisense strand. The ligations can be carried out, for example using blunt end ligation. In some cases, a single molecule consensus sequence for a single template molecule is obtained. A single read from each template molecule can be obtained by comparing the sequence information of the sense and antisense regions.

Sequencing using concatemers of copies of sense and antisense strands

The invention relate to systems and methods for sequencing polynucleotides, as well as detecting reactions and binding events involving other biological molecules. The systems and methods may employ chamber-free devices and nanosensors to detect or characterize such reactions in high-throughput. Because the system in many embodiments is reusable, the system can be subject to more sophisticated and improved engineering, as compared to single use devices.

Systems and methods for genetic and biological analysis

Identifying organisms in a sample using sequencing kinetic signatures

Identification of nucleic acid template-linked barcodes comprising nucleic acid modifications

Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Methods are provided that include linking forward and reverse strands of a double stranded segment to form circular template molecules, and obtaining sequence data from the circular template molecule to identify modified bases, for example including the use of bisulfite treatment. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.

Methods of sequencing and identifying the position of a modified base in a nucleic acid

Growing community of inventors

La Honda, CA, United States of America

Cheryl Heiner