Roche Molecular Systems, Inc.

The Scripps Research Institute

Roche Diagnostics Gmbh

F. Hoffmann-la Roche Ag

Cherie Holcomb

Russell G Higuchi

Nancy J Schoenbrunner

Henry A Erlich

Alison Tsan

Daniel R Salomon

Igor Kozlov

Nick Newton

Bryan Hoglund

Karan Kampani

Sharon Legaspi

Trevor Hryce

Priscilla Moonsamy

Melinda Rastrou

Russell Gene Higuchi

Jennifer Griswold

Alan Casilla

Priya Bellare

Russell Higuchi

Qiao Ruan

Provided herein are methods and components for successive capture of nucleic acids using magnetic glass particles.

Successive capture of nucleic acid by magnetic glass particles

The invention is a method of detecting or assessing solid organ graft (transplant) rejection by detecting donor-specific HLA alleles in a blood sample of a graft (transplant) recipient. The invention further comprises a method of detecting the presence of maternal cells in a blood sample of an offspring.

Non-invasive early detection of solid organ transplant rejection by quantitative analysis of mixtures by deep sequencing of HLA gene amplicons using next generation systems

The invention is a single-tube multiplex assay, capable of simultaneously amplifying, detecting and quantifying multiple nucleic acid targets, using multiple hybridization probes, labeled with the same fluorescent reporter label, but each having a distinct melting temperature. The assay can be further multiplexed with the use of multiple sets of hybridization probes, each set labeled with a separate fluorescent reporter label.

Multiplex quantitative nucleic acid amplification and melting assay

The present invention provides methods of detecting cancer cells by detecting the quantity of SENP1 and/or telomerase in a sample.

SENP1 as a marker for cancer

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Oakland, CA, United States of America

Cherie Holcomb