University of Houston System

Chengzhi Cai

Guoting Qin

Jianhua Gu

Chi Ming Yam

Amit Kumar

Analette I Lopez

Chi-Ming Yam

Fei Yu

Steven Pei

Qingkai Yu

Zhongdang Xiao

Chin Ming Yam

Provided herein are fluorinated compounds having chemical structures: (I) where n is 0 or greater, or (II) where m and n are 0 or greater, or an amino acid, a soluble polymer, an oligo(ethylene glycol), a poly(ethylene glycol), or a carbohydrate each fluorinated with perfluorocarbons having the chemical structure (III) where n is 0 or greater. These fluorinated compounds are utilized in contact lenses to impart lipid-resistant, protein-resistant and biofouling-resistant properties, thus reducing discomfort and infection caused by contact lens wear without changing its transmission characteristics. Also provided is an ophthalmic drug delivery system comprising at least one of the compounds described above embedded in the contact lens and a kit to incorporate the ophthalmic into a contact lens. Methods for incorporating these compounds onto a contact lens without affecting transparency and for use in treating an ophthalmologic-associated condition are provided.

Compositions, methods and kits for treating a contact lens

Provided herein are fluorinated compounds having chemical structures: (I) where n is 0 or greater, or (II) where m and n are 0 or greater, or an amino acid, a soluble polymer, an oligo(ethylene glycol), a poly(ethylene glycol), or a carbohydrate each fluorinated with perfluorocarbons having the chemical structure (III) where n is 0 or greater. These fluorinated compounds are utilized in contact lenses to impart lipid-resistant, protein-resistant and biofouling-resistant properties, thus reducing discomfort and infection caused by contact lens wear without changing its transmission characteristics. Also provided is an ophthalmic drug delivery system comprising at least one of the compounds described above embedded in the contact lens and a kit to incorporate the ophthalmic into a contact lens. Methods for incorporating these compounds onto a contact lens without affecting transparency and for use in treating an ophthalmologic-associated condition are provided. 

According to some embodiments, the present invention provides compositions and methods for making and using multifunctional polymerized liposomes finding relevant application in medical sciences, particularly in bioimaging, diagnostics, drug delivery, and drug formulation. The compositions and methods involve lipids that are both polymerizable and have a 'clickable' group that provides the ability to functionalize via a click reaction with various functional moieties useful for the above-listed applications.

Compositions and methods for making and using multifunctional polymerized liposomes

In an embodiment, the present disclosure pertains to a method and compositions for modification of medical devices, such as indwelling medical devices, implantable catheters, in particular, urinary catheters, to enhance formation of a high density and stable biofilm comprising non-pathogenic organisms for the treatment and prevention of colonization of pathogens leading to device-associated infections, such as urinary tract infections. In some embodiments the present disclosure also relates to a method and compositions for storage and use of the catheters coated with a non-pathogenic biofilm. In some embodiments the present disclosure also pertains to a method and compositions for modification of implantable medical devices with bacterial resistant polymers, and/or antimicrobial agents.

Non-pathogenic biofilms and uses thereof

The present invention is generally directed to nanometric biomolecular arrays and to a novel approaches for the preparation of such nanoarrays, based on binding of biomolecules, such as avidin, to templates generated by lithographically-anodizing biocompatible ultrathin films on silicon substrates using AFM anodization lithography. The present invention is also directed to methods of using such arrays.

Preparation of nanometric arrays of biomolecules on oligo- or poly(ethylene glycol) films on silicon surfaces

A sensor that includes a) a silicon (Si) substrate having a surface; and b) a monolayer of oligoethylene glycol (OEG) bonded to the surface via silicon-carbon bonds. Regions of the OEG monolayer distal to the surface are functionalized with a molecular probe serving as a recognition element for a bioanalyte. A method of making a silicon surface that recognizes a biological specimen includes 1) hydrosilylating with a mixture that includes an oligoethylene glycol (OEG) substituted with an alkene at one end of the OEG and capped at the opposing end of the OEG and an oligoethylene glycol (OEG) substituted with an alkene at one end of the OEG and an alkyne having a protecting group at the opposing end of the OEG and 2) removing the protecting group from the alkyne; and 3) reacting the alkyne with a reagent in a 1,3-dipolar cycloaddition.

Preparation of sensors on oligo- or poly (ethylene glycol) films on silicon surfaces

According to some embodiments, the present invention provides a modified silicone surface for interference to pathogen colonization comprising: an activated silicone layer; a plurality of cross-linking dendrimers adsorbed onto to the activated silicone layer; a plurality of ligand derivatives, each bound to at least one of the plurality of cross-linking dendrimers; and a benign biofilm adhered to the plurality of ligand derivatives. According to some embodiments, the present invention provides a method for making a modified silicone surface for interference to pathogen colonization comprising activating a silicone surface; adsorbing a plurality of cross-linking dendrimers to the silicone surface; binding a plurality of ligand derivatives to the plurality of cross-linking dendrimers; and adhering a benign biofilm to the plurality of ligand derivatives.

Compositions and methods for modifying a silicone surface for prolonged interference against pathogen colonization

A sensor that includes a) a silicon (Si) substrate having a surface; and b) a monolayer of oligoethylene glycol (OEG) bonded to the surface via silicon-carbon bonds. Regions of the OEG monolayer distal to the surface are functionalized with a ligand serving as a recognition element for a bioanalyte. The ligand is covalently bonded in these regions as a cycloadduct of a 1,3-dipolar cycloaddition reaction. A method of making a silicon surface that recognizes a biological specimen includes 1) hydrosilylating with a mixture that includes an oligoethylene glycol (OEG) substituted with an alkene at one end of the OEG and capped at the opposing end of the OEG and an oligoethylene glycol (OEG) substituted with an alkene at one end of the OEG and an alkyne having a protecting group at the opposing end of the OEG and 2) removing the protecting group from the alkyne; and 3) reacting the alkyne with a reagent in a 1,3-dipolar cycloaddition. The reagent in the 1,3-dipolar cycloaddition includes a portion capable of being recognized by a biological specimen.

Thin and short cantilevers possess both a low force constant and a high resonance frequency, thus are highly desirable for atomic force microscope (AFM) imaging and force measurement. According to some embodiments, the invention provides small silicon (Si) cantilevers integrated with a Si tip, for example fabricated from SOI wafers that are used for reducing the variation of thickness of the cantilevers. In one example, the fabrication process provided SOI chips containing 40 silicon cantilevers integrating with an ultra-sharp Si tip. The resolution of images obtained with these tips was much higher than those obtained with the commercial tips, while the force constants were much less, that is, more suitable for imaging soft samples. The availability of such SOI chips greatly facilitates large scale modification of the surfaces of the silicon cantilever tips with a monolayer of oligo(ethylene glycol) derivatives that resist the non-specific interactions with proteins, rendering them most suitable for imaging and measurement of biological samples.

Short and thin silicon cantilever with tip and fabrication thereof

The present invention provides for a practical method of grafting oligo- and/or poly(ethyleneglycol) (OEG and/or PEG) derivatives onto hydrogen-terminated silicon surfaces, including the surfaces of silicon scanning probe microscopy (SPM) tips, by hydrosilylation of OEG and/or PEG-terminated alkenes. This invention is related to the development of silicon-based bio-devices, including biochips, biosensors such as SPM probes, microarrays, micro-fluidic systems, and implantable microdevices. This invention is also a practical method to modify (many) SPM probe tips with OEG/PEG derivatives and to subsequently modify the tip apex with functional single molecules to improve the specificity and resolution of AFM imaging and measurements. The functional molecules include the dendritic adsorbates with a functional group at their focal point and with or without a tripod-shaped framework.

Modification of silicon-containing scanning probe microscopy tips and growth of oligo-or poly (ethylene glycol) films on silicon surfaces through formation of Si-C bonds

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Houston, TX, United States of America

Chengzhi Cai