Anhui Agricultural University

Anhui Wanjiang Vegetable Industry Technology Research Institute Co., Ltd.

Yingshang Shili Ecological Agriculture Technology Co., Ltd.

Chenggang Wang

Xiaoyan Tang

Ying Wang

Ting Li

Zegen Zhang

Shidong Zhu

Jinlong Zhang

Qian Yin

Guohu Chen

Liting Zhang

Xueqing Liu

Jinfeng Hou

Hongwei Wen

Lingyun Yuan

Xingxue Huang

Siwen Wu

A method for obtaining regenerated seedlings ofL. ssp.from embryonic tip tissues, including the following steps. A seed is inoculated to a germination medium for dark culture for 60 h. The testa, root tip, two cotyledons and middle growing point of the resultant germinated seed are removed, and an embryonic tip with a length of 3-5 mm is retained as an explant. The explant is sequentially subjected to low-temperature pre-culture in a pre-culture medium for 36 h, room-temperature shaking culture in a liquid bud induction culture medium for 10 min, and bud induction culture in a bud induction culture medium for 20 d. A regenerated plant with 5-6 leaves is transferred to a rooting medium for rooting culture for about 2 weeks, and a well-rooted plant is collected, and subjected to hardening and transplantation into a filed.

Method for obtaining regenerated seedlings offrom embryonic tip tissue

The present invention provides a method for improving a one-time seedling rate of microspore embryoids of, including the steps of: spraying 6-BA onto a flower bud of a plant; sterilizing the flower bud with alcohol and HgCl; releasing microspores, filtering and centrifuging to obtain purified microspores; diluting the microspores with a NLN medium, subpackaging into culture dishes, and adding phytic acid; finally, subjecting to heat shock treatment and transferring to culture in the dark until embryoids appear, and then conducting shaking culture; transferring the cultured embryos in a cotyledon stage onto a MS medium for differentiation culture, wherein the culture conditions are: 4&#xb0; C., 14 h of illumination by blue-red compound light/day, and 14 days of culture; and then continuing to culture under the condition of 25&#xb0; C. and 14 h of illumination by blue-red compound light/day until seedlings.

Method for improving one-time seedling rate of microspore embryoids of brassica campestris SSP. chinensis makino

Growing community of inventors

Hefei, China

Chenggang Wang