National Yang Ming Chiao Tung University

Cheng-Yeh Huang

Da-Jeng Yao

Cheng-Hsien Liu

Shih-Kang Fan

Wensyang Hsu

Hong-Yuan Huang

Po-Yen Tsai

Po-Huai Shih

A magnetic bead-based digital microfluidic immunoanalysis device and a method thereof are provided, which includes a lower plate, an upper plate disposed above the lower plate, a separating structure therebetween and a magnet disposed on the upper plate or the lower plate. The lower plate includes a first electrode layer including a plurality of channel electrodes with different sizes. A droplet containing few magnetic beads is adapted to be disposed on the lower plate and corresponding to the channel electrodes. The magnet attracts the magnetic beads to approach to the smaller one of the channel electrodes though a magnetic force, and when a voltage is applied to the first electrode layer, the droplet is divided to a detection portion with the magnetic beads and a waste-liquid portion without the magnetic beads respectively corresponding to the smaller one and the larger one of the channel electrodes through a dual-direction electrowetting-on-dielectric force.

Magnetic bead-based digital microfluidic immunoanalysis device and method thereof

Growing community of inventors

Hsinchu, Taiwan

Cheng-Yeh Huang