Beckman Instruments, Inc.

Other

Bio-rad Laboratories, Inc.

Cheng-Ming Liu

Hann-Ping Wang

James C Sternberg

Fu-Tai A Chen

Gerald L Klein

You-Zung Hsieh

Zara Safarian

Eli Grushka

Nida Keo

You-Zung Hseih

Protein mixtures are separated by capillary electrophoresis in a buffer containing one or more amino acids in an amount sufficient to prevent or substantially reduce the degree of protein binding to the wall of the capillary.

Protein separations by capillary electrophoresis using amino

A buffer and method useful for the analysis of glycoproteins by capillary zone electrophoresis. The buffer comprises water, a sugar complexing compound, a base compound for adjusting the pH, and a zwitterionic compound. An embodiment of the buffer comprises sodium borate as the complexing compound, sodium hydroxide as the base, and 3-cyclohexylamino-1-propanesulfonic acid as the zwitterionic compound. In the method, a selected glycoprotein is subjected to capillary zone electrophoresis. The proportion or the amount of the glycoprotein is determined by quantitative analysis of the resulting electropherogram.

Capillary electrophoresis of glycosylated proteins

A method for classifying and typing M-proteins present in a sample of serum, etc. of an individual is disclosed. The method employs an on-capillary electrophoretic immunosubtractive method to analyze and classify identified M-proteins.

On-capillary electrophoretic immunosubtraction for classification and

Processes are provided for pretreating body fluid compositions and subsequently analyzing the pretreated body fluid compositions for analytes of interest. Processes for pretreating the compositions include providing size exclusion gel having a molecular weight fractionation range or a molecular weight exclusion such that the size exclusion gel is capable of excluding or fractionating the analytes of interest, and then causing the composition to contact the size exclusion gel in order to separate the analytes from low molecular weight composition components which interfere with the separation and analysis of the analytes of interest. Processes for analyzing pretreated compositions include electrophoretic methods such as capillary zone electrophoresis which involve the separation and detection of analytes of interest.

Method of sample preparation for urine protein analysis with capillary

A method of quantitating proteins in complex samples using capillary electrophoresis can be used both to determine the concentration of a protein in a sample and to determine total protein concentration in the sample. In general, the method of determining the concentration of a marker protein comprises: (1) adding a known quantity of an internal standard compound to a sample containing at least one protein, the internal standard compound selected from the group consisting of benzoic acid substituted with at least one halogen, producing a detector signal in relation to its concentration, and being capable of electrophoretic separation from the protein; (2) subjecting the sample and the internal standard compound to capillary electrophoresis to separate the protein and the internal standard compound from each other and from other components in the sample; (3) measuring the detector signal produced by the internal standard compound and a detector signal produced by the protein to determine a ratio of protein signal to internal standard signal; and (4) determining the concentration of the protein in the sample from a standard curve of protein concentration versus the ratio of protein signal to internal standard signal. A typical protein measurable by the method is albumin. Typically, measurements are made at 214 nm and the internal standard compound is 2,4-dichlorobenzoic acid.

Use of capillary electrophoresis for quantitating the concentration of

Disclosed herein is a methodology for analyzing isoenzymes using capillary zone electrophoresis ('CZE') techniques. Briefly, an isoenzyme-containing sample and a substrate capable of being catalyzed by said isoenzyme into a reaction product are introduced into a capillary column comprising a buffer. Most preferably, the buffer contains the substrate prior to introduction of the sample into such substrate-buffer. CZE separation techniques are applied to the column such that the isoenzymes are separated from each other into discrete zones. The separation techniques are terminated such that product is rapidly generated by the catalytic conversion of substrate by the isoenzymes, and accumulated, within each discrete zone, followed by detection of product. Information regarding the relative distribution of the isoenzymes can be derived from the relative distribution of the product.

Capillary zone electrophoretic analysis of isoenzymes

Disclosed herein are methodologies for the Capillary Electrophoretic Immunosubtraction ('CEI') of samples. In a preferred embodiment, CEI of a sample comprising constituent parts to be separated comprises the steps of: (1) separating a first aliquot of the sample into constituent parts by capillary electrophoretic techniques, and detecting said parts; (2) admixing a second aliquot of said sample with at least one specific binding partner to a constituent of said sample, said specific binding partner capable of being substantially removed from said aliquot; (3) separating said second aliquot into constituent parts by capillary electrophoretic techniques and detecting said parts; and (4) comparing the separated constituent parts of step (3) with the separated constituent parts of step (1).

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Yorba Linda, CA, United States of America

Cheng-Ming Liu