Jiangnan University

Cheng Lu

Zhengyu Jin

Yaoqi Tian

Xing Zhou

Long Chen

Zhengjun Xie

Jie Long

Yuxiang Bai

Chao Qiu

Xingfei Li

Ziying Ye

Disclosed are an agarase mutant with improved thermal stability and application thereof, belonging to the fields of genetic engineering technology and enzyme engineering. The present disclosure provides an agarase mutant, which is obtained by mutating the amino acid at the 86site, the 373site, the 374site, the 496site, the 507site, or the 747site of agarase with an amino acid sequence as shown in SEQ ID NO. 1. The agarase mutant provided by the present disclosure improves the thermal stability and the hydrolytic activity of the agarase. Compared with the wild type enzyme, the mutant enzyme shows excellent heat resistance and can be industrially used at a relatively high temperature, so that the utilization rate of agar raw materials and the yield of oligosaccharides from agar are improved, and the mutant enzyme has a good industrial application prospect.

Agarase mutant with improved thermal stability and application thereof

Growing community of inventors

Wuxi, China

Cheng Lu