State Grid Jiangsu Electric Power Company

Amphastar Nanjing Pharmaceuticals Inc.

Jiangsu Frontier Power Technology Co., Ltd.

Huawei Cloud Computing Technologies Co., Ltd.

Nanjing Chscom Electrical Technology Co., Ltd.

Jiangsu Keneng Electric Power Engineering Consulting Co., Ltd.

Cheng Li

Hao Chen

Haoning Zhang

Song Chen

Chuangen Tang

Yong Xia

Ning Wang

Yaohong Li

Yujun Lu

Jiajia Cao

Shangshu Pan

Xiaorui Liu

Huaiyan Cui

Chengliang Wang

Yonggao Ge

Hongxing Wang

Li Zhang

Zhenyu Chen

Xiaofeng Yang

Haifeng Li

Xiao Lu

Jijun Yin

Jianyu Luo

Jie Fan

Ting Zhou

Xiaofeng Wang

Dongmei Xie

Jeffrey Ding

Junxia Qian

Jinhu Wang

Feng Ye

Kaifang Ding

Taotao Liu

Zhong Sheng

Provided are a novel pro-insulin aspart structure design and a method for preparing the pro-insulin aspart. The method comprises designing the pro-insulin aspart sequence, constructing a recombinant insulin aspart engineered bacteria, inducing expression of an insulin fusion protein as an inclusion body in the engineered bacteria, and extracting the insulin fusion protein from the engineered bacteria by means of denaturing, renaturing enzymatic digestion, and separation purification operations. By modifying the recombinant leading peptide and C-peptide sequences, the method avoids the need to include a cyanogen bromide cleavage step. In some embodiments, the C-peptide is replaced by a single amino acid which tends to reduce quality loss associated with enzymatic digestion.

Pro-insulin aspart structure and method for preparing insulin aspart

This application provides a trace chain information query method, including: receiving, by a trace chain server, first trace chain information sent by a first service node and second trace chain information sent by a second service node, where the first service node is a service node in a first trace chain, the second service node is a service node in a second trace chain, both the first trace chain and the second trace chain are generated as triggered by a same user operation, the first trace chain information includes a group identifier, the second trace chain information includes the group identifier, and the group identifier is used to indicate the user operation; and finding, by the trace chain server, the first trace chain information and the second trace chain information based on the group identifier.

Trace chain information query method and device

The provided materials are a grid load interactive control method and terminal based on adaptive load characteristics and a storage medium. The method includes: receiving a test presetting message sent by a master station and sending message reply information to the master station; parsing the test presetting message to obtain a test presetting instruction; building a test environment and generating a virtual action exit in the test environment when the test presetting instruction generates a record; and sending instruction response including the record to the master station so as to cause the master station to determine whether a test is successful according to the record.

Grid load interactive control method and terminal based on adaptive load characteristics, and storage medium

Provided is a battery energy storage grid-load interactive method, terminal, system and medium for superimposed control. The method includes: receiving, by the battery energy storage grid-load interactive terminal, a load shedding instruction sent by a master station; sending, by the battery energy storage grid-load interactive terminal, the load shedding instruction to the power conversion system to enable the power conversion system to switch the operating state of the power conversion system from a charging state or a standby state or a discharging state to a maximum power discharging state according to the load shedding instruction; and sending, by the battery energy storage grid-load interactive terminal, the load shedding instruction to the energy managing system to enable the energy managing system to control the power conversion system.

Battery energy storage grid-load interactive method, terminal, system and medium for superimposed control

The invention belongs to the technical field of polypeptide preparation methods, and in particular relates to a preparation method of a liraglutide intermediate polypeptide GLP-1 (7-37). In the preparation method, main steps include constructing recombinant liraglutide engineered bacteria viato induce expression of a liraglutide intermediate fusion protein in the form of inclusion bodies, and performing denaturation, renaturation, enzyme digestion, separation and purification to obtain the liraglutide intermediate polypeptide GLP-1 (7-37). The invention alters expression pattern into the expression of the intracellular insoluble inclusion bodies by changing a signal peptide of the recombinant sequence to increase significantly expression level. The liraglutide intermediate polypeptide prepared by the invention has a purity up to 87% or more and a yield of more than 85%.

Method for preparing liraglutide intermediate polypeptide

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Jiangsu, China

Cheng Li