Tsinghua University

Raydium Semiconductor Corporation

Other

National Yang Ming Chiao Tung University

Taipei Medical University

Benq Materials Corporation

Crystalvue Medical Corporation

Cheng-Hsien Liu

Chung-Cheng Chou

Long Hsu

Fung-Hsu Wu

Chen Peng

William Wang

Ta-Yuan Lee

Sheng-Yang Tseng

Da-Jeng Yao

Shih-Kang Fan

Huang-Ming Philip Chen

Jen-Tsan Ashley Chi

Wai William Wang

Sung-Yang Wei

Hwan-You Chang

Hong-Yuan Huang

Pei-Yu Chang

Chang-Hung Hsieh

Wensyang Hsu

Wen-Syang Hsu

Hsin-Yu Lai

Chen-Wei Wu

Hui-Ling Lin

Chang-Hung Tien

Yu-Chen Chen

Ting-Ju Yueh

Hung-Yu Yeh

Chi-Fan Chen

Chung-Yu Chou

Kuo-Wei Chang

Ting-Chou Wei

Ai-Tang Chang

Cheng-En Liu

Yuh-Cherng Lai

Yun-Ting Yao

Wei-Chia Chang

Chin-Jung Li

Bing-Ying Ho

Yu-Ting Tai

Yu-Ching Tung

Te-Yu Chao

Han-Jung Liao

Shu-Chuan Ho

Kang-Yun Lee

Mao-Chih Hsieh

Cheng-Yeh Huang

Weilun Sun

Po-Chen Shih

Po-Yen Tsai

Chau-Ting Yeh

Ting-Sheng Shih

Po-Huai Shih

Tzu-Chi Yu

An array platform for three-dimensional cell culturing and drug testing and screening is disclosed. In the array platform, a hydrogel-cell mixture injection area is configured to inject a plurality of kinds of hydrogel-cell mixtures. Cell observation areas are connected to the hydrogel-cell mixture injection area. Electrodes are disposed under the cell observation areas and automatic cell quantification and three-dimensional cell co-arrangement of the plurality of kinds of hydrogel-cell mixtures in the cell observation areas through the electrodes to imitate a structure of body's tissues. A drug injection area is configured to inject a plurality of kinds of drugs. Drug combination generators respectively correspond to the cell observation areas and are connected to the drug injection area. Each drug combination generator has a microfluidic channel structure and configured to generate drug combinations according to the plurality of kinds of drugs.

Array platform for three-dimensional cell culturing and drug testing and screening

A drug screening platform simulating hyperthermic intraperitoneal chemotherapy including a dielectrophoresis system, a microfluidic chip and a heating system is disclosed. The dielectrophoresis system is used to provide a dielectrophoresis force. The microfluidic chip includes a cell culture array and observation module and a drug mixing module. The cell culture array and observation module are used to arrange the cells into a three-dimensional structure through the dielectrophoresis force to construct a three-dimensional tumor microenvironment. The drug mixing module is coupled to the cell culture array and observation module and used to automatically split and mix the inputted drugs and output the drug combinations into the cell culture array and observation module. The heating system is used for real-time temperature sensing and heating control of the drug combinations on the microfluidic chip to simulate high-temperature drug environment when performing hyperthermic intraperitoneal chemotherapy on the three-dimensional tumor microenvironment.

Drug screening platform simulating hyperthermic intraperitoneal chemotherapy

A portable ring-type fluorescence optical system for observing microfluidic channel and an operating method thereof are disclosed. The portable ring-type fluorescence optical system includes a photographic chip, a first polarizer, an objective lens, a ring-type fluorescent light source, a biological sample on a microfluidic chip, a second polarizer and a bottom illumination light source arranged in order from top to bottom. The ring-type fluorescent light source is used to generate a ring-type fluorescent light to the biological sample on the microfluidic chip. The objective lens is used to magnify a fluorescent image of the biological sample on the microfluidic chip to focus on the photographic chip. The first polarizer disposed under the photographic chip and the second polarizer disposed under the biological sample form a non-zero angle to each other to block reflected lights that the biological sample reflects the lights emitted by the bottom illumination light source.

Portable ring-type fluorescence optical system for observing microfluidic channel and operating method thereof

An optical measuring apparatus and an operating method thereof are disclosed. The optical measuring apparatus includes a light source, a carrier chip, a light sensor, an analyzing chip and a display. Samples are uniformly distributed on the carrier chip. The light source emits sensing lights toward the carrier chip. The light sensor receives the sensing lights passing through the carrier chip at a plurality of times to obtain a plurality of images corresponding to the plurality of times respectively. The analyzing chip is coupled to the light sensor. The analyzing chip analyzes the object number and distribution variation with time in the sample according to the plurality of images corresponding to the plurality of times and estimates intrinsic characteristics of the object in the sample accordingly. The display is coupled to the analyzing chip. The display displays the intrinsic characteristics of the object in the sample.

Optical measuring apparatus and operating method thereof

A magnetic bead-based digital microfluidic immunoanalysis device and a method thereof are provided, which includes a lower plate, an upper plate disposed above the lower plate, a separating structure therebetween and a magnet disposed on the upper plate or the lower plate. The lower plate includes a first electrode layer including a plurality of channel electrodes with different sizes. A droplet containing few magnetic beads is adapted to be disposed on the lower plate and corresponding to the channel electrodes. The magnet attracts the magnetic beads to approach to the smaller one of the channel electrodes though a magnetic force, and when a voltage is applied to the first electrode layer, the droplet is divided to a detection portion with the magnetic beads and a waste-liquid portion without the magnetic beads respectively corresponding to the smaller one and the larger one of the channel electrodes through a dual-direction electrowetting-on-dielectric force.

Magnetic bead-based digital microfluidic immunoanalysis device and method thereof

The present disclosure illustrates a platform system for in vitro cell co-cultivation with automatic trapping function. The platform system aims to develop a bio-chip applied in cell culture systems, and has several features. The first feature is that this co-cultivation platform can construct a micro environment suitable for culture of various cells. The second feature is dynamic perfusion. The microfluidic system is used to dynamically replace the culture medium, in order to maintain an appropriate environment for the growth of cells. The third feature is the automatic trapping. The cells to be cultured can be trapped in a suitable location according to the flow resistance, so that the damaged on the cell caused by manual operation can be minimized.

Platform system for in vitro cell co-cultivation with automatic trapping function

Disclosed is a microfluidic chip and method using the same. The microfluidic chip comprises a substrate having a surface, and at least a tissue culture area formed on the surface of the substrate. The tissue culture area has a microfluidic channel formed by a plurality of connected geometrical structures (nozzle-type channels) having a predetermined depth. The microfluidic channel has an inlet and an outlet, which are at two ends of the microfluidic channel, for medium inputting and outputting, respectively. Additionally, at least an air-exchange hole is formed on the bottom of the microfluidic channel. By using the microfluidic chip for tissue culture, lateral flow speed and stress can be decreased, so as to prolong survival time of tissues (e.g. liver tissues).

Microfluidic chip and method using the same

An apparatus of generating an optical tweezers with momentum and method thereof and an optical tweezers photo-image for guiding particles are provided. The apparatus generates at least one optical tweezers on an examined object that carries at least one particle. The apparatus includes a laser source, a diffractive optical element and a convergent lens. The laser beam from the laser source passes through the diffractive optical element to produce a diffractive pattern. The laser beam is then received by the convergent lens and then to be focused on a plane of the examined object. The optic axis of the convergent lens is substantially not perpendicular to the plane of the examined object, so that the laser beam is projected onto the plane of the examined object in a skewed manner for providing a lateral momentum to move the particle.

Apparatus of generating an optical tweezers with momentum and method thereof and photo-image for guiding particles

An apparatus of generating optical tweezers with momentum is provided for providing optical tweezers with a first momentum on a test piece. The apparatus comprises a laser source, a diffractive optical element (DOE) and a lens. The laser source is for outputting a laser beam. The DOE has a first phase-delay picture, and the laser beam forms a diffraction pattern after passing the first phase-delay picture. The lens is for receiving and focusing the diffraction pattern on the test piece to form the first optical tweezers with the first momentum. The lens has an optical axis intersecting the DOE at an optical intersection point, a geometric center of the phase-delay picture has a displacement vector relative to the optical intersection point and a direction of the first momentum is related to a direction of the displacement vector.

Apparatus and method of generating optical tweezers with momentum

A disk structure is disposed in an optical tweezers device including a light source for producing incident laser light. The disk structure includes a first substrate, a second substrate and a reflective layer. The second substrate is disposed with respect to the first substrate. One of the first substrate and the second substrate has at least one flow path. The reflective layer, which is adhered to the second substrate, is disposed between the first substrate and the second substrate. After the incident laser light passes through the first substrate and then reaches the reflective layer, the incident laser light is reflected back as reflective laser light by the reflective layer to form reflective laser light. A tweezers light field is formed in the flow path by both the reflective laser light and the incident laser light.

Disk structure, manufacturing method thereof and optical tweezers device using the same

An optical tweezers lifting apparatus is provided. The optical tweezers lifting apparatus includes an optical tweezers and a particle-lifting device. The particle-lifting device includes a substrate and a plurality of electrodes that are disposed on the bottom of a flow path in the substrate. When a dielectrophoresis (DEP) solution with a plurality of floating particles is conducted into the flow path and upon those electrodes and a voltage is applied to these electrodes, these particles would be driven by a negative DEP force to move upward to a specific depth in the flow path. Meanwhile, the optical tweezers of the apparatus is selectively focused at the specific depth in the flow path.

Optical tweezers lifting apparatus

An optical tweezers controlling device including a light source, an objective lens and a focus adjusting unit is provided. The focus adjusting unit disposed between the light source and the objective lens includes a mirror set and a zoom lens set. The mirror set has at least a mirror. The mirror is rotatable such that after a light of the light source is projected to the mirror, the reflective direction of the light reflected from the mirror is changeable. The zoom lens set has at least a zoom lens disposed in accordance with the mirror. By rotating the mirror or changing the focal length of the zoom lens, the focusing location of the light changes on the focal plane of the objective lens or in the front or the rear of the focal plane.

Optical tweezers controlling device

An apparatus and a method for changing optical tweezers are provided. The apparatus includes a diffractive optical element (DOE), a mask unit and an objective lens. The DOE includes a plurality of phase delay patterns. The mask unit includes a plurality of mask patterns that correspond to the phase delay patterns, respectively, wherein at least a portion of the mask patterns are complementary. A laser beam passing through each phase diffractive pattern correspondingly passes through each mask pattern to generate a compound diffractive pattern. The objective lens receives the compound diffractive pattern and focuses it on an examining object to form an optical tweezers.

Apparatus and method for changing optical tweezers

Growing community of inventors

Hsinchu, Taiwan

Cheng-Hsien Liu