Pacific Biosciences of California, Inc.

Illumina Corporation

Mgi Tech Co., Ltd.

Cheng Frank Zhong

Paul M Lundquist

Hovig Bayandorian

Jonas Korlach

Dietrich A Dehlinger

Mathieu Foquet

Hod Finkelstein

Shifeng Li

Jian Gong

Yan-You Lin

Juraj Topolancik

Stephen Wilson Turner

Boyan Boyanov

Darren Segale

Liang Wang

Denis Zaccarin

Peiqian Zhao

Eliane H Trepagnier

Yanqiao Huang

Annette Claire Grot

Pezhman Monadgemi

Ravi Saxena

Aaron J Rulison

Paul Lundquist

Joy Roy

Chen Li

John Dixon

Yu Liu

Kevin Hester

Yiwen Ouyang

Mathieu Foguet

Yujuan Cheng

An apparatus for forming a plurality of microdroplets () from a droplet () includes a substrate (), a dielectric layer () on the substrate () and having a plurality of hydrophilic surface regions () spaced apart from each other by a hydrophobic surface (), and a plurality of electrodes () in the dielectric layer (). The electrodes () are configured to form an electric field (E) across the droplet () in response to voltages provided by a control circuit () to move the droplet () across the dielectric layer () in lateral direction (F) while leaving portions of the droplet () on the hydrophilic surface regions () to form the plurality of microdroplets () on the hydrophilic surface regions ().

Apparatus and method for forming droplets having predetermined volume by electrowetting

An apparatus for forming a plurality of microdroplets from a droplet includes a substrate, a dielectric layer on the substrate and having a plurality of hydrophilic surface regions spaced apart from each other by a hydrophobic surface, and a plurality of electrodes covered by the dielectric layer. The electrodes are configured to form an electric field across the droplet in response to voltages provided by a control circuit to move the droplet across the dielectric layer in a lateral direction while leaving portions of the droplet on the hydrophilic surface regions to form the plurality of microdroplets on the hydrophilic surface regions.

Method for manufacturing an apparatus for manipulating a droplet

Biosensor including a device base having a sensor array of light sensors and a guide array of light guides. The light guides have input regions that are configured to receive excitation light and light emissions generated by biological or chemical substances. The light guides extend into the device base toward corresponding light sensors and have a filter material. The device base includes device circuitry electrically coupled to the light sensors and configured to transmit data signals. A passivation layer extends over the device base and forms an array of reaction recesses above the light guides. The biosensor also includes peripheral crosstalk shields that at least partially surround corresponding light guides of the guide array to reduce optical crosstalk between adjacent light sensors.

Biosensors for biological or chemical analysis and methods of manufacturing the same

An integrated detection, flow cell and photonics (DFP) device is provided that comprises a substrate having an array of pixel elements that sense photons during active periods. The substrate and pixel elements form an IC photon detection layer. At least one wave guide is formed on the IC photo detection layer as a photonics layer. An optical isolation layer is formed over at least a portion of the wave guide. A collection of photo resist (PR) walls patterned to define at least one flow cell channel that is configured to direct fluid along a fluid flow path. The wave guides align to extend along the fluid flow path. The flow cell channel is configured to receive samples at sample sites that align with the array of pixel elements.

Method and system for fluorescence lifetime based sequencing

A microfluidic apparatus () can include a PCB (), a biological chip () overlying the PCB (), and a microfluidic housing () overlying the biological chip () and the PCB (). The microfluidic apparatus () also has a first adhesive layer () attaching the microfluidic housing () to the biological chip () and a second adhesive layer () attaching the microfluidic housing () to the PCB (). The second adhesive layer () is thicker than the first adhesive layer (). The first adhesive layer () comprises a first adhesive material, and the second adhesive layer () comprises a second adhesive material.

System and method for integrated sensor cartridge

Embodiments of the invention provide an improved biosensor for biological or chemical analysis. According to embodiments of the invention, backside illumination (BSI) complementary metal-oxide-semiconductor (CMOS) image sensors can be used to effectively analyze and measure fluorescence or chemiluminescence of a sample. This measured value can be used to help identify a sample. Embodiments of the invention also provide methods of manufacturing an improved biosensor for biological or chemical analysis and systems and methods of DNA sequencing.

A method for forming sequencing flow cells can include providing a semiconductor wafer covered with a dielectric layer and forming a patterned layer on the dielectric layer. The patterned layer has a differential surface that includes alternating first surface regions and second surface regions. The method can also include attaching a cover wafer to the semiconductor wafer to form a composite wafer structure including a plurality of flow cells. The composite wafer structure can then be singulated to form a plurality of dies. Each die forms a sequencing flow cell. The sequencing flow cell can include a flow channel between a portion of the patterned layer and a portion of the cover wafer, an inlet, and an outlet. Further, the method can include functionalizing the sequencing flow cell to create differential surfaces.

Wafer level sequencing flow cell fabrication

This invention provides devices for use in various analytical applications including single-molecule analytical reactions. Methods for detecting analytes optically by propagating optical energy by waveguides within a substrate are provided. Analytical devices are provided which have both shallow and deep waveguides in which illumination light is transported through the deep waveguides and coupled into the shallow waveguides. The shallow waveguides provide evanescent field illumination to analytes, such as single-molecule analytes, within nanometer scale wells. Integrated devices including integrated detectors such as CMOS detectors are included.

Substrates and optical systems and methods of use thereof

Multilayer electrical connection for digital microfluidics on substrates

A device for luminescent imaging includes an array of imaging pixels, a photonic structure over the array of imaging pixels, and an array of features over the photonic structure. A first feature of the array of features is over a first pixel of the array of imaging pixels, and a second feature of the array of features is over the first pixel and spatially displaced from the first feature. A first luminophore is within or over the first feature, and a second luminophore is within or over the second feature. The device includes a radiation source to generate first photons having a first characteristic at a first time, and generate second photons having a second characteristic at a second time. The first pixel selectively receives luminescence emitted by the first and second luminophores responsive to the first photons at the first time and second photons at the second time, respectively.

Photonic structure-based devices and compositions for use in luminescent imaging of multiple sites within a pixel, and methods of using the same

Analytic device comprising a substrate, nanometer-scale wells, and shallow waveguide optically coupled to a deep waveguide

A method for forming sequencing flow cells can include providing a semiconductor wafer covered with a dielectric layer, and forming a patterned layer on the dielectric layer. The patterned layer has a differential surface that includes alternating first surface regions and second surface regions. The method can also include attaching a cover wafer to the semiconductor wafer to form a composite wafer structure including a plurality of flow cells. The composite wafer structure can then be singulated to form a plurality of dies. Each die forms a sequencing flow cell. The sequencing flow cell can include a flow channel between a portion of the patterned layer and a portion of the cover wafer, an inlet, and an outlet. Further, the method can include functionalizing the sequencing flow cell to create differential surfaces.

Biosensors for biological or chemical analysis and methods of manufacturing the same comprising a plurality of wells formed by a metal or metal oxide layer

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Fremont, CA, United States of America

Cheng Frank Zhong