Abbvie Inc.

Millipore Corporation

Emd Millipore Corporation

Chen Wang

Nanying Bian

Neil P Soice

Joaquin A Umana

Chris Chumsae

Germano Coppola

Kwok-Shun Cheng

John Charkoudian

Senthilkumar Ramaswamy

Brian Gagnon

Dennis Aquino

Andrew Lyddiatt

Senthil Ramaswamy

Yuan Wong

Lyddiatt Andrew

Senthikumar Ramaswamy

Disclosed herein are compositions and methods for the isolation and purification of proteins from a sample. In particular, the present invention relates to compositions and methods for isolating and purifying proteins incorporating a displacement chromatographic step. The present invention is also directed toward pharmaceutical compositions comprising one or more antibodies purified by a method described herein.

Protein purification using displacement chromatography

The present invention relates to low acidic species (AR) compositions comprising a protein, e.g., an antibody, or antigen-binding portion thereof, and methods for producing such low AR compositions using displacement chromatography. Methods for using such compositions to treat a disorder, e.g., a disorder in which TNF&#x3b1; is detrimental, are also provided.

Low acidic species compositions and methods for producing and using the same using displacement chromatography

The invention relates generally to solid supports for chromatography. In specific embodiments the invention provides for solid supports suitable for affinity chromatography along with methods, systems and kits which use the same.

Media for affinity chromatography

The present invention relates to an asymmetric chromatography media suitable for separations applications, particularly as packed bed, fluidized bed or magnetized bed chromatography media. In certain embodiments, the asymmetric chromatography media comprises asymmetric particles, preferably beads, having at least two distinct, controlled pore size distributions. Preferably one of the distinct pore size distributions is in an internal region of the particle, and the other is in an external region or coating on the particle. These distinct pore size distributions can be modified with uniform or alternatively unique functional groups or mixtures of functional groups. The present invention allows for the control over pore size distribution within an asymmetric porous particle by providing a distinct internal region, preferably in the form of a bead, and a distinct external region, preferably in the form of a coating on the bead.

Asymmetric porous adsorptive bead

The invention provides methods of coupling protein ligands to a solid support. The invention also provides affinity chromatography matrices and methods of using affinity chromatography matrices to purify a target molecule.

Affinity chromatography matrices and methods of making and using the same

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Acton, MA, United States of America

Chen Wang