Life Technologies Corporation

Applied Biosystems, LLC

Pierce Biotechnology, Inc.

Life Technologies Holdings Pte Limited

Chee Kiong Lim

Kok Siong Teo

Gary A Chappell

Chee Wee Ching

Hon Siu Shin

Scott Rickes

Sean Zimmerman

Michael C Pallas

Kuan Moon Boo

Soo Yong Lau

Jew Kwee Ngui

Gregory John Kilmer

Yang Hooi Kee

Lim Hi Tan

Way Xuang Lee

John Langan

Ching Ong Lau

Thomas Conner

Chin Yong Koo

Kevin Albert Maher

Jacob K Freudenthal

Wuh Ken Loh

Gary Lim

Francis T Cheng

Jorge Fonseca

Siew Ying Koh

Tiong Han Toh

Woon Liang Soh

Ronald Danehy

Ming Shen

David Fortescue

Niroshan Ramachandran

Ming Song Chen

Ruoyun Wu

Geoffrey Dahlhoff

Ted Straub

Jeff Dere

Thiam Chye Lee

Thomas A Conner

Hon Siu No 23 Tampines Street 34 Shin

A biological analysis system is provided. The system comprises an interchangeable assembly configured to accommodate any one of a plurality of sample holders, each respective sample holder configured to receive a plurality of samples. The system also includes a control system configured to cycle the plurality of samples through a series of temperatures. The system further includes an optical system configured to detect fluorescent signals emitted from the plurality of samples. The optical system, in particular, can comprise a single field lens, an excitation source, an optical sensor, and a plurality of filter components. The excitation source can be one or more light emitting diodes. The field lends can be a bi-convex lens.

Systems and methods for biological analysis

Blot and gel imaging instrument display screen with graphical user interface

A thermal block assembly including a sample block and two or more thermoelectric devices, is disclosed. The sample block has a top surface configured to receive a plurality of reaction vessels and an opposing bottom surface. The thermoelectric devices are operably coupled to the sample block, wherein each thermoelectric device includes a housing for a thermal sensor and a thermal control interface with a controller. Each thermoelectric device is further configured to operate independently from each other to provide a substantially uniform temperature profile throughout the sample block.

Apparatuses, systems and methods for providing thermocycler thermal uniformity

Aspects of the present teachings describe a method and apparatus for automatically controlling a block temperature to reduce undershooting and overshooting of the temperatures of a sample contained in the block and participating in a polymerase chain reaction (PCR). The adaptive thermal block temperature control begins when a sample temperature enters a sample window region between a preliminary setpoint temperature and a target setpoint temperature for the sample. Based on thermodynamic behavior of the sample and the predetermined phase of PCR, predicting a time period measured subsequent to the preliminary setpoint temperature when the sample will reach the target setpoint suitable for the predetermined phase of PCR. During this time period, varying the block temperature ramp rate with a series of cooling and heating changes to ensure the block temperature reaches the target setpoint temperature at approximately the same time as the sample reaches the same. Synchronizing the block temperature and sample temperature to the target setpoint temperature reduces undershooting and overshooting of the sample temperature and increases the speed and efficiency of the overall PCR process as it relates to the thermal cycling operations.

Adaptive thermal block temperature control method and system

In one aspect, a thermal cycler system including a sample block and a thermoelectric device is disclosed. In various embodiments, the sample block has a first surface configured to receive a plurality of reaction vessels and an opposing second surface. In various embodiments the thermoelectric device is operably coupled to the second surface of the sample block. In various embodiments a thermal control unit is provided. In various embodiments the thermal control unit includes a computer processing unit. In various embodiments the thermal control unit includes an electrical current source. In various embodiments the thermal control unit also includes an electrical interface portion configured to connect the thermoelectric device with the electrical current source by way of an electrical cable. In various embodiments the thermal control unit is oriented in a different plane than the sample block and thermoelectric cooler.

Apparatuses, systems and methods for providing scalable thermal cyclers and isolating thermoelectric devices

A method for performing polymerase chain reactions (PCR) for improving thermal non-uniformity is provided. The method includes measuring a first temperature, by a first sensor, of a first sample block sector of a sample block and measuring a second temperature, by a second sensor, of a second sample block sector of the sample block that is adjacent to the first sample block sector. The method further includes calculating, by a thermoelectric controller, a difference in temperature between the first temperature and the second temperature and adjusting, by the thermoelectric controller, the first temperature of the first sample block sector based on the difference in temperature by using one or more thermoelectric coolers. The one or more thermoelectric coolers is configured to heat or cool the first sample block sector by adjusting power output from the thermoelectric controller.

Thermal uniformity for thermal cycler instrumentation using dynamic control

An apparatus and method for rapid thermal cycling including a thermal diffusivity plate. The thermal diffusivity plate can provide substantial temperature uniformity throughout the thermal block assembly during thermal cycling by a thermoelectric module. An edge heater can provide substantial temperature uniformity throughout the thermal block assembly during thermal cycling.

Thermal cycling apparatus and method for providing thermal uniformity

Display screen with graphical user interface

Graphical user interface for a display screen

Growing community of inventors

Singapore, Singapore

Chee Kiong Lim